Developmental neurotoxicity of antimony (Sb) in the early life stages of zebrafish

• Published in: Ecotoxicology and environmental safety Vol. 218; p. 112308
• Main Authors: Xia, Siyu, Zhu, Xinhong, Yan, Yuepei, Zhang, Tao, Chen, Guoliang, Lei, Daoxi, Wang, Guixue
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 15.07.2021; Elsevier
• Subjects: AChE; Behavior; Danio rerio; Oxidative stress; Toxicity; AChE; Oxidative stress; Behavior; Toxicity; Danio rerio
• ISSN: 0147-6513; 1090-2414; 1090-2414
• DOI: 10.1016/j.ecoenv.2021.112308

Accumulating studies have revealed the toxicity of antimony (Sb) to soil-dwelling and aquatic organisms at the individual level. However, little is known about the neurotoxic effects of antimony and its underlying mechanisms. To assess this issue, we investigated the neurotoxicity of antimony (0, 200, 400, 600 and 800 mg/L) in zebrafish embryos. After exposure, zebrafish embryos showed abnormal phenotypes such as a shortened body length, morphological malformations, and weakened heart function. Behavioral experiments indicated that antimony caused neurotoxicity in zebrafish embryos, manifested in a decreased spontaneous movement frequency, delayed response to touch, and reduced movement distance. We also showed that antimony caused a decrease in acetylcholinesterase (AChE) levels in zebrafish embryos, along with decreased expression of neurofunctional markers such as gfap, nestin, mbp, and shha. Additionally, antimony significantly increased reactive oxygen species levels and significantly reduced glutathione (GSH) and superoxide dismutase (SOD) activity. In summary, our findings indicated that antimony can induce developmental toxicity and neurotoxicity in zebrafish embryos by affecting neurotransmitter systems and oxidative stress, thus altering behavior. These outcomes will advance our understanding of antimony-induced neurotoxicity, environmental problems, and health hazards. •Antimony affects neurobehavior in the early life stages of zebrafish.•Antimony induces oxidative stress and decreases AChE activity.•Antimony decreases the astrocyte viability in zebrafish larvae.•Antimony alters the expression of the neurofunctional marker genes.