Do initial concentration and activated sludge seasonality affect pharmaceutical biotransformation rate constants?

• Published in: Applied microbiology and biotechnology Vol. 105; no. 16-17; pp. 6515 - 6527
• Main Authors: van Bergen, Tamara J. H. M., Rios-Miguel, Ana B., Nolte, Tom M., Ragas, Ad M. J., van Zelm, Rosalie, Graumans, Martien, Scheepers, Paul T. J., Jetten, Mike S. M., Hendriks, A. Jan, Welte, Cornelia U.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.08.2021; Springer Nature B.V
• Subjects: Acetaminophen; Activated sludge; Aquatic environment; Biodegradability; Biodegradation; Biological activity; Biomedical and Life Sciences; Biotechnology; Biotransformation; Carbamazepine; Chromatography, Liquid; Community composition; Composition; Diclofenac; Drugs; Environmental Biotechnology; Environmental risk; Fluoxetine; Gene sequencing; Inoculum; Kinetic theory; Life Sciences; Metformin; Metoprolol; Microbial activity; Microbial Genetics and Genomics; Microbiology; Microorganisms; Pharmaceutical Preparations; Pharmaceuticals; Rate constants; RNA, Ribosomal, 16S - genetics; rRNA 16S; Seasonal variations; Sewage; Sludge; Summer; Tandem Mass Spectrometry; Terbutaline; Wastewater treatment; Wastewater treatment plants; Water Pollutants, Chemical - analysis; Winter; Sorption; Organic micropollutants; Kinetics; Wastewater treatment plants; Bacterial community; Nitrification
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-021-11475-9

Pharmaceuticals find their way to the aquatic environment via wastewater treatment plants (WWTPs). Biotransformation plays an important role in mitigating environmental risks; however, a mechanistic understanding of involved processes is limited. The aim of this study was to evaluate potential relationships between first-order biotransformation rate constants ( k b ) of nine pharmaceuticals and initial concentration of the selected compounds, and sampling season of the used activated sludge inocula. Four-day bottle experiments were performed with activated sludge from WWTP Groesbeek (The Netherlands) of two different seasons, summer and winter, spiked with two environmentally relevant concentrations (3 and 30 nM) of pharmaceuticals. Concentrations of the compounds were measured by LC–MS/MS, microbial community composition was assessed by 16S rRNA gene amplicon sequencing, and k b values were calculated. The biodegradable pharmaceuticals were acetaminophen, metformin, metoprolol, terbutaline, and phenazone (ranked from high to low biotransformation rates). Carbamazepine, diatrizoic acid, diclofenac, and fluoxetine were not converted. Summer and winter inocula did not show significant differences in microbial community composition, but resulted in a slightly different k b for some pharmaceuticals. Likely microbial activity was responsible instead of community composition. In the same inoculum, different k b values were measured, depending on initial concentration. In general, biodegradable compounds had a higher k b when the initial concentration was higher. This demonstrates that Michealis-Menten kinetic theory has shortcomings for some pharmaceuticals at low, environmentally relevant concentrations and that the pharmaceutical concentration should be taken into account when measuring the k b in order to reliably predict the fate of pharmaceuticals in the WWTP. Key points • Biotransformation and sorption of pharmaceuticals were assessed in activated sludge. • Higher initial concentrations resulted in higher biotransformation rate constants for biodegradable pharmaceuticals. • Summer and winter inocula produced slightly different biotransformation rate constants although microbial community composition did not significantly change. Graphical abstract