Evaluation of three commercial serological tests with different methodologies to assess Helicobacter pylori infection

The sera of 142 Helicobacter pylori-positive and 32 H. pylori-negative patients were assessed by a desktop test (QuickVue), an enzyme-linked immunosorbent assay (ELISA) (HM-CAP), and a solid-phase, two-step chemiluminescent enzyme immunoassay (Immulite). These tests yielded sensitivities of 97, 97,...

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Published inJournal of clinical microbiology Vol. 37; no. 12; pp. 4150 - 4152
Main Authors VAN DER ENDE, A, VAN DER HULST, R. W. M, ROORDA, P, TYTGAT, G. N. J, DANKERT, J
Format Journal Article
LanguageEnglish
Published Washington, DC American Society for Microbiology 01.12.1999
SeriesNote
Subjects
Adult
Antibodies, Bacterial - blood
Bacterial diseases
Bacterial diseases of the digestive system and abdomen
Bacteriology
Biological and medical sciences
Endoscopy
Enzyme-Linked Immunosorbent Assay
Evaluation Studies as Topic
Gastritis - microbiology
Helicobacter Infections - diagnosis
Helicobacter Infections - immunology
Helicobacter Infections - microbiology
Helicobacter pylori
Helicobacter pylori - immunology
Human bacterial diseases
Humans
Immunoenzyme Techniques
Infectious diseases
Luminescent Measurements
Medical sciences
Peptic Ulcer - microbiology
Predictive Value of Tests
Sensitivity and Specificity
Serologic Tests
Performance evaluation
Human
Spirillales
Spirillaceae
Serology
Enzyme immunoassay
Infection
Helicobacter pylori
Bacteriosis
Digestive diseases
Bacteria
Diagnosis
ELISA assay
Comparative study
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Summary:The sera of 142 Helicobacter pylori-positive and 32 H. pylori-negative patients were assessed by a desktop test (QuickVue), an enzyme-linked immunosorbent assay (ELISA) (HM-CAP), and a solid-phase, two-step chemiluminescent enzyme immunoassay (Immulite). These tests yielded sensitivities of 97, 97, and 91% and specificities of 97, 94, and 100%, respectively. In conclusion, the desktop test and the ELISA are more sensitive than the chemiluminescent enzyme immunoassay (P < 0.05). The chemiluminescent enzyme immunoassay has the advantage that it is fully automated.
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Present address: Kennemer Gasthuis, Department of Internal Medicine, Gastroenterology, 2023 EA Haarlem, The Netherlands.
Corresponding author. Mailing address: Department of Medical Microbiology, Academic Medical Center, University of Amsterdam, P.O. Box 22700, 1100 DE Amsterdam, The Netherlands. Phone: 31-20-5664862. Fax: 31-20-6979271.
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.37.12.4150-4152.1999