Relationship between genome packaging and Gag translation/AUG of primate lentiviruses
About the relationship between retroviral genome packaging and translation, three possible modes (random-, trans-, and cis-) of packaging process could be assumed. In this report, we developed an assay system based on the RT-qPCR to measure the packaging efficiency of primate lentiviruses. With this...
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Published in | Microbes and infection Vol. 21; no. 2; pp. 119 - 123 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
France
Elsevier Masson SAS
01.03.2019
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Subjects | |
Online Access | Get full text |
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Summary: | About the relationship between retroviral genome packaging and translation, three possible modes (random-, trans-, and cis-) of packaging process could be assumed. In this report, we developed an assay system based on the RT-qPCR to measure the packaging efficiency of primate lentiviruses. With this system, we analyzed the genome packaging modes of primate lentiviruses such as HIV-1, 2, SIVmac and SIVagm. The data suggested that the modes of all viruses analyzed were very similar. In addition, we observed that the Gag-AUG sequences of them played important roles for maintaining efficient packaging, other than the initiation of translation. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1286-4579 1769-714X |
DOI: | 10.1016/j.micinf.2018.09.002 |