Relationship between genome packaging and Gag translation/AUG of primate lentiviruses

About the relationship between retroviral genome packaging and translation, three possible modes (random-, trans-, and cis-) of packaging process could be assumed. In this report, we developed an assay system based on the RT-qPCR to measure the packaging efficiency of primate lentiviruses. With this...

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Bibliographic Details
Published inMicrobes and infection Vol. 21; no. 2; pp. 119 - 123
Main Authors Sakuragi, Sayuri, Shioda, Tatsuo, Sakuragi, Jun-ichi
Format Journal Article
LanguageEnglish
Published France Elsevier Masson SAS 01.03.2019
Subjects
Codon, Initiator - genetics
Gene Products, gag - genetics
Genome dimerization
HEK293 Cells
HIV
HIV-1 - genetics
HIV-1 - physiology
HIV-2 - genetics
HIV-2 - physiology
Humans
Lentivirus
Lentiviruses, Primate - genetics
Lentiviruses, Primate - physiology
Mutation
Packaging
Protein Biosynthesis
Simian Immunodeficiency Virus - genetics
Simian Immunodeficiency Virus - physiology
SIV
Translation
Virus Assembly - genetics
Packaging
SIV
Genome dimerization
Translation
HIV
Lentivirus
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Summary:About the relationship between retroviral genome packaging and translation, three possible modes (random-, trans-, and cis-) of packaging process could be assumed. In this report, we developed an assay system based on the RT-qPCR to measure the packaging efficiency of primate lentiviruses. With this system, we analyzed the genome packaging modes of primate lentiviruses such as HIV-1, 2, SIVmac and SIVagm. The data suggested that the modes of all viruses analyzed were very similar. In addition, we observed that the Gag-AUG sequences of them played important roles for maintaining efficient packaging, other than the initiation of translation.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:1286-4579
1769-714X
DOI:10.1016/j.micinf.2018.09.002