Cytokinesis-block micronucleus cytome (CBMN-CYT) assay biomarkers and telomere length analysis in relation to inorganic elements in individuals exposed to welding fumes

• Published in: Ecotoxicology and environmental safety Vol. 212; p. 111935
• Main Authors: Quintana-Sosa, Milton, León-Mejía, Grethel, Luna-Carrascal, Jaime, De moya, Yurina Sh, Rodríguez, Ibeth Luna, Acosta-Hoyos, Antonio, Anaya-Romero, Marco, Trindade, Cristiano, Narváez, Diana María, Restrepo, Helena Groot de, Dias, Johnny, Niekraszewicz, Liana, Garcia, Ana Letícia Hilario, Rohr, Paula, da Silva, Juliana, Henriques, João Antonio Pêgas
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 01.04.2021; Elsevier
• Subjects: Air Pollutants, Occupational - analysis; Biological Assay; Biomarkers - analysis; Cytokinesis; Cytome; DNA Damage; Humans; Inorganic elements; Lymphocytes; Micronuclei; Micronucleus Tests - methods; Occupational Exposure - analysis; Oxidative Stress; Telomere; Welding; Welding; Inorganic elements; Cytome; Telomere; Micronuclei
• ISSN: 0147-6513; 1090-2414
• DOI: 10.1016/j.ecoenv.2021.111935

During the welding activities many compounds are released, several of these cause oxidative stress and inflammation and some are considered carcinogenic, in fact the International Agency for Research on Cancer established that welding fumes are carcinogenic to humans. The aim of the present study was to analyze the cytotoxic and genotoxic potential of exposure to welding fumes and to determine concentrations of metals in blood and urine of occupationally exposed workers. We included 98 welders and 100 non-exposed individuals. Our results show significant increase in the frequency of micronuclei (MN), nucleoplasmic bridges (NPB), nuclear buds (NBUD) and necrotic cells (NECR) in cytokinesis-block micronucleus cytome (CBMN-Cyt) assay, as well as in the telomere length (TL) of the exposed individuals with respect to the non-exposed group. In the analysis of the concentrations of inorganic elements using PIXE method, were found higher concentrations of Cr, Fe and Cu in the urine, and Cr, Fe, Mg, Al, S, and Mn in the blood in the exposed group compared to the non-exposed group. A significant correlation was observed between MN and age and between NPB and years of exposure. Additionally, we found a significant correlation for TL in relation to MN, NPB, age and years of exposure in the exposed group. Interestingly, a significant correlation between MN and the increase in the concentration of Mg, S, Fe and Cu in blood samples of the exposed group, and between MN and Cr, Fe, Ni and Cu in urine. Thus, our findings may be associated with oxidative and inflammatory damage processes generated by the components contained in welding fumes, suggesting a high occupational risk in welding workers. [Display omitted] •Welding fumes are carcinogenic to humans.•Cytotoxic and genotoxic effects in welders were investigated.•Correlation between TL and genetic instability was found.•Higher concentrations of several metals were found in blood and urine.•Occupational risk for welders was demonstrated.