Bioprospecting of indigenous biosurfactant-producing oleophilic bacteria for green remediation: an eco-sustainable approach for the management of petroleum contaminated soil

• Published in: 3 Biotech Vol. 12; no. 1; p. 13
• Main Authors: Bharali, Pranjal, Bashir, Yasir, Ray, Anggana, Dutta, Nipu, Mudoi, Pronab, Alemtoshi, Sorhie, Viphrezolie, Vishwakarma, Vinita, Debnath, Palash, Konwar, Bolin Kumar
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.01.2022; Springer Nature B.V
• Subjects: Agriculture; Aromatic compounds; Bacteria; Biodegradation; Bioinformatics; Biomaterials; Bioprospecting; Bioremediation; Biosurfactants; Biotechnology; Cancer Research; Chemistry; Chemistry and Materials Science; Consortia; Crude oil; Fractions; Gas chromatography; Hydrocarbons; Micelles; Molecular weight; Original; Original Article; Performance degradation; Phenanthrene; Pollutants; Polyaromatic hydrocarbons; Polycyclic aromatic hydrocarbons; Pseudomonas aeruginosa; Sludge; Soil contamination; Soil pollution; Soil remediation; Soils; Stem Cells; Strains (organisms); Sulfur; Surfactants; Biosurfactant; Critical micelle concentration (CMC); Consortium; Hydrocarbon; Pseudomonas aeruginosa
• ISSN: 2190-572X; 2190-5738
• DOI: 10.1007/s13205-021-03068-0

In the present study, the efficiency of four different strains of Pseudomonas aeruginosa and their biosurfactants in the bioremediation process were investigated. The strains were found to be capable of metabolizing a wide range of hydrocarbons (HCs) with preference for high molecular weight aliphatic (ALP) over aromatic (ARO) compounds. After treating with individual bacteria and 11 different consortia, the residual crude oils were quantified and qualitatively analyzed. The bacterial strains degraded ALP, ARO, and nitrogen, sulphur, oxygen (NSO) containing fractions of the crude oil by 73–67.5, 31.8–12.3 and 14.7–7.3%, respectively. Additionally, the viscosity of the residual crude oil reduced from 48.7 to 34.6–39 mPa s. Further, consortium designated as 7 and 11 improved the degradation of ALP, ARO, and NSO HCs portions by 80.4–78.6, 42.7–42.4 and 21.6–19.2%, respectively. Moreover, addition of biosurfactant further increased the degradation performance of consortia by 81.6–80.7, 43.8–42.6 and 22.5–20.7%, respectively. Gas chromatographic analysis confirmed the ability of the individual strains and their consortium to degrade various fractions of crude oil. Experiments with biosurfactants revealed that polyaromatic hydrocarbons (PAHs) are more soluble in the presence of biosurfactants. Phenanthrene had the highest solubility among the tested PAHs, which further increased as biosurfactant doses raised above their respective critical micelle concentrations (CMC). Furthermore, biosurfactants were able to recover 73.5–63.4% of residual oil from the sludge within their respective CMCs. Hence, selected surfactant-producing bacteria and their consortium could be useful in developing a greener and eco-sustainable way for removing crude oil pollutants from soil.