Immunophenotyping and oncogene amplifications in tumors of the papilla of Vater

• Published in: Virchows Archiv : an international journal of pathology Vol. 453; no. 6; pp. 579 - 588
• Main Authors: Baumhoer, Daniel, Zlobec, Inti, Tornillo, Luigi, Dietmaier, Wolfgang, Wuensch, Peter H., Hartmann, Arndt, Sessa, Fausto, Ruemmele, Petra, Terracciano, Luigi M.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.12.2008; Springer; Springer Nature B.V
• Subjects: Adenoma; Adenoma - genetics; Adenoma - metabolism; Adenoma - pathology; Adolescent; Adult; Aged; Aged, 80 and over; Ampulla of Vater; Ampulla of Vater - metabolism; Ampulla of Vater - pathology; Bax protein; bcl-2-Associated X Protein - genetics; bcl-2-Associated X Protein - metabolism; Biological and medical sciences; Biomarkers, Tumor - genetics; Biomarkers, Tumor - metabolism; c-Myc protein; Carcinoma; Common Bile Duct Neoplasms - genetics; Common Bile Duct Neoplasms - metabolism; Common Bile Duct Neoplasms - pathology; Cyclin D1 - genetics; Cyclin D1 - metabolism; Endoscopy; Ephrin-B2 - genetics; Ephrin-B2 - metabolism; ErbB-2 protein; Female; Fluorescence in situ hybridization; Gastroenterology. Liver. Pancreas. Abdomen; Gene Amplification; Gene Expression Regulation, Neoplastic; Humans; Immunohistochemistry; Immunophenotyping; Keratin-20 - genetics; Keratin-20 - metabolism; Keratin-7 - genetics; Keratin-7 - metabolism; Liver. Biliary tract. Portal circulation. Exocrine pancreas; Male; Medical sciences; Medicine; Medicine & Public Health; Middle Aged; Mucosa; Oncogenes; Original Article; Pathology; Proliferating Cell Nuclear Antigen - genetics; Proliferating Cell Nuclear Antigen - metabolism; Proto-Oncogene Proteins c-myc - genetics; Proto-Oncogene Proteins c-myc - metabolism; Receptor, ErbB-2 - genetics; Receptor, ErbB-2 - metabolism; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus; Tumors; Young Adult; Amplification; FISH; Ampullary carcinoma; TMA; CCND1; Papilla of Vater; Human; Immunophenotype; Cyclin D1; Ampullary carcinoma . Papilla of Vater; Gene amplification; Ampulla of Vater cancer; Immunological investigation; C-Onc gene; Genetics; Digestive diseases; Intestinal disease; Protooncogene; Pancreatic disease
• ISSN: 0945-6317; 1432-2307
• DOI: 10.1007/s00428-008-0669-7

Carcinomas of the ampulla of Vater are rare and assumed to generally arise from preexisting adenomas (adenoma–carcinoma sequence). Histologically, distinct subtypes can be distinguished that were shown to differ significantly in terms of clinical outcome. Since pathologists usually receive bioptic tissue samples of ampullary tumors obtained during endoscopy, accurate classification of carcinoma subtypes can sometimes be difficult on morphological criteria alone. We therefore performed immunohistochemistry using a panel of established marker proteins (CK7, CK20, p21, p27, ESA, bax, and ephrin-B2) on 175 carcinoma, 111 adenoma, and 152 normal mucosa specimens of the ampulla of Vater and identified distinct immunoprofiles for every carcinoma subtype. Fluorescence in situ hybridization analyses of therapeutic target genes (c-myc, EGFR1, CCND1, HER2) found CCND1 to represent the most frequently amplified gene in our series (7.5%).