Bactericidal Activity of Copper-Zinc Hybrid Nanoparticles on Copper-Tolerant Xanthomonas perforans

• Published in: Scientific reports Vol. 9; no. 1; pp. 20124 - 9
• Main Authors: Carvalho, Renato, Duman, Kamil, Jones, Jeffrey B., Paret, Mathews L.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 27.12.2019; Nature Publishing Group
• Subjects: 140/133; 631/326/22/1434; 631/326/421; Adaptation, Biological; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Antimicrobial activity; Bacteria; Bactericidal activity; Bactericides; Biodegradation; Copper; Copper - chemistry; Drug Resistance, Bacterial; Humanities and Social Sciences; Lycopersicon esculentum - microbiology; Metal Nanoparticles - chemistry; multidisciplinary; Nanoparticles; Phenotype; Phytotoxicity; Pigments, Biological; Plant Diseases - microbiology; Raman spectroscopy; Science; Science (multidisciplinary); Spot; Strains (organisms); Xanthomonas; Xanthomonas - drug effects; Xanthomonas - metabolism; Xanthomonas perforans; Zinc - chemistry
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-019-56419-6

Bacterial spot of tomato, caused by Xanthomonas perforans , X. euvesicatoria , X. vesicatoria and X. gardneri , is a major disease, contributing to significant yield losses worldwide. Over dependence of conventional copper bactericides over the last decades has led to the prevalence of copper-tolerant strains of Xanthomonas spp., making copper bactericides ineffective. Thus, there is a critical need to develop new strategies for better management of copper-tolerant Xanthomonas spp. In this study, we investigated the antimicrobial activity of a hybrid nanoparticle, copper-zinc (Cu/Zn), on copper-tolerant and sensitive strains. The hybrid nanoparticle significantly reduced bacterial growth in vitro compared to the non-treated and micron-size commercial copper controls. Tomato transplants treated with the hybrid nanoparticle had significantly reduced disease severity compared to the controls, and no phytotoxicity was observed on plants. We also studied the hybrid nanoparticle effect on the bacterial pigment xanthomonadin using Near-Infra Red Raman spectroscopy as an indicator of bacterial degradation. The hybrid nanoparticle significantly affected the ability of X. perforans in its production of xanthomonadin when compared with samples treated with micron-size copper or untreated. This study sheds new light on the potential utilization of this novel multi-site Cu/Zn hybrid nanoparticle for bacterial spot management.