Transfer of antibiotic resistance genes from soil to rice in paddy field

• Published in: Environment international Vol. 191; p. 108956
• Main Authors: Xu, Min, Wang, Fang, Stedtfeld, Robert D., Fu, Yuhao, Xiang, Leilei, Sheng, Hongjie, Li, Zhongpei, Hashsham, Syed A., Jiang, Xin, Tiedje, James M.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 01.09.2024; Elsevier
• Subjects: Agriculture; Animals; Antibiotic resistance genes; Composting; Drug Resistance, Microbial - genetics; Fertilization; Fertilizers; Genes, Bacterial; Oryza - genetics; Paddy field; Red soil; Soil - chemistry; Soil Microbiology; Swine; Transfer; Transfer; Paddy field; Fertilization; Antibiotic resistance genes; Red soil
• ISSN: 0160-4120; 1873-6750; 1873-6750
• DOI: 10.1016/j.envint.2024.108956

•Rice seedling plays an important role in the import of ARGs in paddy field.•Application of fertilizer shows little influence on ARGs profile of rice grains in the field.•Manure-borne ARGs have the potential to transfer from soil to rice plant.•ARGs in rice seedling have the potential to transfer into soil and rice grains. The global spread and distribution of antibiotic resistance genes (ARGs) has received much attention whereas knowledge about the transmission of ARGs from one matrix to another is still insufficient. In this study, the paddy fields fertilized with chemical fertilizer, swine compost, and no fertilizer were investigated to assess the transfer of ARGs from soil to rice. Soil and plant samples were collected at day 0, 7, 30 and 79 representing various stages of paddy growth. High throughput qPCR was applied to quantify ARGs using a set of 144 primers. Gene copy number of ARGs measured in soil initially decreased and then increased in soil with no fertilizer and chemical fertilizer, indicating that crop planting and flooding conditions did influence the ARGs profiles in soil. Application of swine compost significantly enhanced the relative abundance and gene copy number of ARGs in paddy soil. Rice seedlings contained substantial amount of ARGs and their relative abundance continually decreased after transplant. Compared with initial stage, detection frequencies of ARGs increased in soil without swine compost at harvest time (day 79), indicating the transmission of ARGs from irrigation water to soil. Detection frequencies of ARGs increased in soil and rice root with swine compost at harvest time, indicating the transfer of ARGs from swine compost to soil and rice root. There was no significant difference in abundance and diversity of ARGs in rice grains with these three different fertilizations. The source of the ARGs in rice grain still needs further exploration.