Banana starch nanocomposite with cellulose nanofibers isolated from banana peel by enzymatic treatment: In vitro cytotoxicity assessment

• Published in: Carbohydrate polymers Vol. 207; pp. 169 - 179
• Main Authors: Tibolla, H., Pelissari, F.M., Martins, J.T., Lanzoni, E.M., Vicente, A.A., Menegalli, F.C., Cunha, R.L.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.03.2019; Elsevier 1
• Subjects: Caco-2 Cells; Cell Survival - drug effects; Cellulose - chemistry; Cellulose - toxicity; Cellulose nanofibers; Elastic Modulus; Enzymatic hydrolysis; Food Packaging - instrumentation; Fruit - chemistry; Humans; Hydrolysis; Musa - chemistry; Nanocomposites - chemistry; Nanocomposites - toxicity; Nanofiber cytotoxicity; Nanofibers - chemistry; Nanofibers - toxicity; Permeability; Science & Technology; Starch - chemistry; Starch - toxicity; Starch composite; Tensile Strength; Water - chemistry; Starch composite; Nanofiber cytotoxicity; Enzymatic hydrolysis; Cellulose nanofibers
• ISSN: 0144-8617; 1879-1344; 0144-8617
• DOI: 10.1016/j.carbpol.2018.11.079

[Display omitted] •Cellulose nanofibers from banana peel were incorporated in banana starch-based films.•Cellulose nanofibers showed no cytotoxic effect on the Caco-2 until 2000 μg/mL.•CNFs improved the mechanical, optical and water barrier properties of the starch film.•The nanocomposites properties were strongly influenced by CNFs aspect ratio.•Banana peel CNFs are potentially applicable as reinforcement in polymeric matrices. The potential use of cellulose nanofibers (CNFs) as a reinforcing agent in banana starch-based nanocomposite films was investigated. CNFs were isolated from banana peel (Musa paradisiaca) by enzymatic hydrolysis. Banana starch-based nanocomposite films were prepared with CNFs using the casting method. CNFs effect on cell viability and on nanocomposite films properties’ was investigated. The cytotoxicity of CNFs was assessed on Caco-2 cell line. CNFs were not cytotoxic at 50–2000 μg/mL. However, CNFs above 2000 μg/mL significantly decreased cell viability. Topography analysis showed that the incorporation of CNFs modified the film structure. The nanocomposites exhibited a complex structure due to strong interactions between CNFs and starch matrix, promoting a remarkable improvement on mechanical and water barrier properties, opacity and UV light barrier compared to the control film. CNFs can offer a great potential as reinforcing material for starch-based nanocomposite films, producing a value-added food packaging from a waste material.