Stability studies with tigecycline in bacterial growth medium and impact of stabilizing agents

• Published in: European journal of clinical microbiology & infectious diseases Vol. 40; no. 1; pp. 215 - 218
• Main Authors: Amann, Lisa F., Vicente, Emilia Ruda, Rathke, Mareike, Broeker, Astrid, Riedner, Maria, Wicha, Sebastian G.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.01.2021; Springer Nature B.V
• Subjects: Antibacterial activity; Ascorbic acid; Bacteria; Biodegradation; Biomedical and Life Sciences; Biomedicine; Brief Report; Degradation; Internal Medicine; Medical Microbiology; Pyruvic acid; Stability analysis; Stabilizers (agents); Supplements; Tigecycline; Degradation; Mueller Hinton broth; Stability; Time-kill studies; Tigecycline
• ISSN: 0934-9723; 1435-4373
• DOI: 10.1007/s10096-020-03970-0

Purpose This study aimed to examine the degradation of tigecycline in Mueller Hinton broth (ca-MHB), as knowledge about bacterial susceptibility is key for therapeutic decisions. Methods Antioxidative stabilizers were evaluated on tigecycline stability in a quantitative chromatography assay and tigecycline induced kill against Staphylococcus aureus (ATCC29213) was determined in time kill studies. Results Ascorbic acid caused rapid degradation of tigecycline and resulted in loss of antibacterial activity. Tigecycline was stabilized in aged broth by 2% pyruvate and bacterial growth, and tigecycline killing was similar to fresh broth without supplementation, but independent of age. Conclusion Our results underline the importance of using freshly prepared ca-MHB or the need for stabilizers for tigecycline susceptibility testing while using aged ca-MHB.