Intermediate and low abundant protein analysis of vitamin D deficient obese and non-obese subjects by MALDI-profiling

• Published in: Scientific reports Vol. 7; no. 1; pp. 12633 - 10
• Main Authors: Al-Daghri, Nasser M., Torretta, Enrica, Capitanio, Daniele, Fania, Chiara, Guerini, Franca Rosa, Sabico, Shaun B., Clerici, Mario, Gelfi, Cecilia
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 03.10.2017; Nature Publishing Group
• Subjects: 692/163/2743/393; 692/53/2422; 82; 82/16; 82/29; 82/47; 82/58; 82/80; Apolipoproteins; Cardiovascular diseases; Cholesterol; Complement component C3; Diabetes mellitus; Environmental factors; Humanities and Social Sciences; Lipid metabolism; Low density lipoprotein; Mass spectrometry; Mass spectroscopy; Metabolic disorders; Metabolic syndrome; multidisciplinary; Obesity; Peptides; Proteins; Science; Science (multidisciplinary); Scientific imaging; Triglycerides; Vitamin D; Vitamin deficiency
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-017-13020-z

Obesity is a pathological condition caused by genetic and environmental factors, including vitamin D deficiency, which increases the risk of developing cardiovascular disorders and diabetes. This case-control study was designed to verify whether serum profiles could be identified differentiating obese and non-obese Saudis characterized by vitamin D deficiency and pathological levels of triglycerides, high-density lipoprotein cholesterol and high total cholesterol levels. The serum protein profiles of 64 vitamin D deficient (serum 25(OH)D < 50nmol/L) individuals with metabolic syndrome and with (n = 31; BMI ≥ 30) or without (n = 33; BMI < 30) obesity were analyzed by a quantitative label-free mass spectrometry approach (MALDI-profiling), combined with different serum immunodepletion strategies (Human7 and Human14 immuno-chromatographies), to analyze the intermediate- and low-abundant protein components. The analysis of intermediate-abundant proteins (Human7) in obese vs . non-obese subjects identified 14 changed peaks (p < 0.05) in the m/z range 1500–35000. Furthermore, the Human14 depletion provided new profiles related to obesity (121 changed peaks). Among changed peaks, 11 were identified in the m/z range 1500–4000 Da by high-resolution tandem mass spectrometry, belonging to apolipoprotein CIII, apolipoprotein B100, alpha-1-antichymotrypsin and complement C3. Data herein show that distinct protein profiles identify specific peptides belonging to lipid metabolism and inflammation processes that are associated with obesity and vitamin D deficiency.