RNA extraction from various recalcitrant plant tissues with a cethyltrimethylammonium bromide-containing buffer followed by an acid guanidium thiocyanate-phenol-chloroform treatment

• Published in: Bioscience, biotechnology, and biochemistry Vol. 72; no. 7; pp. 1951 - 1953
• Main Authors: Suzuki, Y.(Tohoku Univ., Sendai (Japan)), Mae, T, Makino, A
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 01.07.2008; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: ARN; Biological and medical sciences; Bis-Trimethylammonium Compounds; Buffers; Chloroform; ELECTROFORESIS; ELECTROPHORESE; ELECTROPHORESIS; EXTRACCION; EXTRACTION; FEUILLE; FLEUR; FLORES; FLOWERS; Fundamental and applied biological sciences. Psychology; Guanidines; HOJAS; LEAVES; Methods; ORGANE DE RESERVE; ORGANOS DE RESERVA; PCR; Phenol; Plant Structures - chemistry; PLANT TISSUES; POLISACARIDOS; POLYHOLOSIDE; POLYSACCHARIDES; RNA; RNA extraction; RNA, Plant - isolation & purification; STORAGE ORGANS; storage tissues; TEJIDOS VEGETALES; Thiocyanates; TISSU VEGETAL; tree leaves; Plant tissue; Flower; flowers; Chloroform; RNA; tree leaves; Plant leaf; RNA extraction; Phenol; Storage; Treatment; storage tissues; Phenols; Thiocyanates; Extraction

High-quality total RNA was extracted using a cethyltrimethylammonium bromide-containing buffer followed by an acid guanidium thiocyanate-phenol-chloroform treatment from recalcitrant plant tissues such as tree leaves (pine, Norway spruce, ginkgo, Japanese cedar, rose), flowers (rose, Lotus japonicus...