RNA extraction from various recalcitrant plant tissues with a cethyltrimethylammonium bromide-containing buffer followed by an acid guanidium thiocyanate-phenol-chloroform treatment

• Published in: Bioscience, biotechnology, and biochemistry Vol. 72; no. 7; pp. 1951 - 1953
• Main Authors: Suzuki, Y.(Tohoku Univ., Sendai (Japan)), Mae, T, Makino, A
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 01.07.2008; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: ARN; Biological and medical sciences; Bis-Trimethylammonium Compounds; Buffers; Chloroform; ELECTROFORESIS; ELECTROPHORESE; ELECTROPHORESIS; EXTRACCION; EXTRACTION; FEUILLE; FLEUR; FLORES; FLOWERS; Fundamental and applied biological sciences. Psychology; Guanidines; HOJAS; LEAVES; Methods; ORGANE DE RESERVE; ORGANOS DE RESERVA; PCR; Phenol; Plant Structures - chemistry; PLANT TISSUES; POLISACARIDOS; POLYHOLOSIDE; POLYSACCHARIDES; RNA; RNA extraction; RNA, Plant - isolation & purification; STORAGE ORGANS; storage tissues; TEJIDOS VEGETALES; Thiocyanates; TISSU VEGETAL; tree leaves; Plant tissue; Flower; flowers; Chloroform; RNA; tree leaves; Plant leaf; RNA extraction; Phenol; Storage; Treatment; storage tissues; Phenols; Thiocyanates; Extraction
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.80084

High-quality total RNA was extracted using a cethyltrimethylammonium bromide-containing buffer followed by an acid guanidium thiocyanate-phenol-chloroform treatment from recalcitrant plant tissues such as tree leaves (pine, Norway spruce, ginkgo, Japanese cedar, rose), flowers (rose, Lotus japonicus) and storage tissues (seeds of Lotus japonicus and rice, sweet potato tuber, banana fruit). This protocol greatly reduced the time required for RNA extraction.