Effect of hydrolyzed collagen from defatted Asian sea bass (Lates calcarifer) skin on fibroblast proliferation, migration and antioxidant activities

Hydrolyzed collagen from the defatted Asian sea bass ( Lates calcarifer ) (Asbs-HC) had high hydrophobic amino acids and imino acids. When fibroblast cell was treated with Asbs-HC, there was no cytotoxicity at any concentrations (25–1000 µg/mL). Asbs-HC at 1000 µg/mL exhibited the highest cell proli...

Full description

Saved in:
Bibliographic Details
Published inJournal of food science and technology Vol. 58; no. 2; pp. 541 - 551
Main Authors Chotphruethipong, Lalita, Sukketsiri, Wanida, Aluko, Rotimi E., Sae-leaw, Thanasak, Benjakul, Soottawat
Format Journal Article
LanguageEnglish
Published New Delhi Springer India 01.02.2021
Springer Nature B.V
Subjects
Amino acids
Antioxidants
Cell adhesion & migration
Cell migration
Cell proliferation
Chelation
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
Collagen
Cytotoxicity
Fibroblasts
Food Science
Hydrophobicity
Lates calcarifer
Molecular weight
Nutrition
Original
Original Article
Peptides
Scavenging
Sea bass
Toxicity
Wound healing
Defatting
Hydrolyzed collagen
Antioxidant
Bioactive peptides
Wound-healing
Fibroblast proliferation
Online AccessGet full text

Cover

More Information
Summary:Hydrolyzed collagen from the defatted Asian sea bass ( Lates calcarifer ) (Asbs-HC) had high hydrophobic amino acids and imino acids. When fibroblast cell was treated with Asbs-HC, there was no cytotoxicity at any concentrations (25–1000 µg/mL). Asbs-HC at 1000 µg/mL exhibited the highest cell proliferation and cell migration ( p  < 0.05), indicating wound healing ability. Antioxidative activities of Asbs-HC at different concentrations were determined. ABTS radical scavenging activity (ABTS-RSA) and oxygen radical absorbance capacity (ORAC) increased when Asbs-HC levels augmented up to 1 mg/mL ( p  < 0.05). Decreased activities in scavenging DPPH radical and chelating metal were found at higher levels of Asbs-HC (0.5 and 1 mg/mL) ( p  < 0.05). Molecular weight (MW) of peptides in Asbs-HC ranged from 406 to 16,120 Da. Peptide containing MW of 406 Da rendered the highest scavenging activity towards ABTS radical. Thus, Asbs-HC could be applied as antioxidant, skin nourishment and wound healing agents for food/drink fortification.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0022-1155
0975-8402
DOI:10.1007/s13197-020-04566-4