The use of innovative and efficient nanocomposite (magnetic graphene oxide) for the reduction on of Fusarium mycotoxins in palm kernel cake

• Published in: Scientific reports Vol. 7; no. 1; pp. 12453 - 9
• Main Authors: Pirouz, A. A., Selamat, J., Iqbal, S. Z., Mirhosseini, H., Karjiban, R. Abedi, Bakar, F. Abu
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 29.09.2017; Nature Publishing Group
• Subjects: 631/326/193; 639/638/899; Adsorbents; Adsorption; Animal Feed - analysis; Animals; Deoxynivalenol; Factor Analysis, Statistical; Feeds; Food contamination & poisoning; Food Contamination - analysis; Food science; Fumonisin B1; Fumonisins - isolation & purification; Fusarium; Fusarium - chemistry; Fusarium - pathogenicity; Graphene; Graphite - chemistry; Humanities and Social Sciences; Hydrogen-Ion Concentration; Liquid chromatography; Magnetite Nanoparticles - chemistry; Magnetite Nanoparticles - ultrastructure; Mass spectrometry; Mass spectroscopy; Metabolites; multidisciplinary; Mycotoxins; Nanocomposites; Nanocomposites - chemistry; Nanocomposites - ultrastructure; Palm Oil; pH effects; Plant Oils - chemistry; Regression analysis; Science; Science (multidisciplinary); Solvents; Temperature; Toxins; Trichothecenes; Trichothecenes - isolation & purification; Zearalenone; Zearalenone - isolation & purification
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-017-12341-3

Adsorption plays an important role in the removal of mycotoxins from feedstuffs. The main objective of this study was to investigate the efficacy of using magnetic graphene oxide nanocomposites (MGO) as an adsorbent for the reduction of Fusarium mycotoxins in naturally contaminated palm kernel cake (PKC). Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to assess the mycotoxins in animal feed. Target mycotoxins included the zearalenone (ZEA), the fumonisins (FB 1 and FB 2 ) and trichothecenes (deoxynivalenol (DON), HT-2 and T-2 toxin). Response surface methodology (RSM) was applied to investigate the effects of time (3–7 h), temperature (30–50 °C) and pH (3–7) on the reduction. The response surface models with (R 2  = 0.94–0.99) were significantly fitted to predict mycotoxins in contaminated PKC. Furthermore, the method ensured a satisfactory adjustment of the polynomial regression models with the experimental data except for fumonisin B 1 and B 2 , which decrease the adsorption of magnetic graphene oxide (MGO). The optimum reduction was performed at pH 6.2 for 5.2 h at of 40.6 °C. Under these optimum conditions, reduced levels of 69.57, 67.28, 57.40 and 37.17%, were achieved for DON, ZEA, HT-2, and T-2, respectively.