Rbpj direct regulation of Atoh7 transcription in the embryonic mouse retina
In vertebrate retinal progenitor cells, the proneural factor Atoh7 exhibits a dynamic tissue and cellular expression pattern. Although the resulting Atoh7 retinal lineage contains all seven major cell types, only retinal ganglion cells require Atoh7 for proper differentiation. Such specificity neces...
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Published in | Scientific reports Vol. 8; no. 1; pp. 10195 - 13 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
London
Nature Publishing Group UK
05.07.2018
Nature Publishing Group |
Subjects | |
Online Access | Get full text |
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Summary: | In vertebrate retinal progenitor cells, the proneural factor
Atoh7
exhibits a dynamic tissue and cellular expression pattern. Although the resulting
Atoh7
retinal lineage contains all seven major cell types, only retinal ganglion cells require
Atoh7
for proper differentiation. Such specificity necessitates complex regulation of
Atoh7
transcription during retina development. The Notch signaling pathway is an evolutionarily conserved suppressor of proneural bHLH factor expression. Previous
in vivo
mouse genetic studies established the cell autonomous suppression of
Atoh7
transcription by
Notch1
,
Rbpj
and
Hes1
. Here we identify four CSL binding sites within the
Atoh7
proximal regulatory region and demonstrate Rbpj protein interaction at these sequences by
in vitro
electromobility shift, calorimetry and luciferase assays and,
in vivo
via colocalization and chromatin immunoprecipitation. We found that Rbpj simultaneously represses
Atoh7 t
ranscription using both Notch-dependent and –independent pathways. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 2045-2322 2045-2322 |
DOI: | 10.1038/s41598-018-28420-y |