Polystyrene micro-/nanoplastics induced hematopoietic damages via the crosstalk of gut microbiota, metabolites, and cytokines

• Published in: Environment international Vol. 161; p. 107131
• Main Authors: Jing, Jiaru, Zhang, Lei, Han, Lin, Wang, Jingyu, Zhang, Wei, Liu, Ziyan, Gao, Ai
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 01.03.2022; Elsevier
• Subjects: Animals; Cytokines; Gastrointestinal Microbiome; Gut microbiota; Hemopoietic toxicity; Metabolites; Mice; Mice, Inbred C57BL; Microplastics; Polystyrenes - toxicity; RNA, Ribosomal, 16S; Gut microbiota; MIP-1beta; PS; Cytokines; BFU-E; LT-HSCs; MPP; TCDCA; OTU; CFU-GEMM; Hemopoietic toxicity; Metabolites; MNPLs; CFU-GM; KC; HSC; Microplastics; ST-HSCs
• ISSN: 0160-4120; 1873-6750
• DOI: 10.1016/j.envint.2022.107131

[Display omitted] •Hematopoietic damage caused by polystyrene micro-/nanoplastics was confirmed.•MNPLs caused the disorder of gut microbiota, metabolites, and cytokines.•The flora-metabolite-cytokine axes mediated hematopoietic damage induced by MNPLs. Micro-/nanoplastics (MNPLs), novel environmental pollutants, widely exist in the environment and life and bring health risks. Previous studies have shown that NMPLs can penetrate bone marrow, but whether they cause hematopoietic damage remains uncertain. In this study, C57BL/6J mice were treated with polystyrene MNPLs (PS-MNPLs, 10 μm, 5 μm and 80 nm) at 60 μg doses for 42 days by intragastric administration. We evaluated the hematopoietic toxicity induced by MNPLs and potential mechanisms via combining 16S rRNA, metabolomics, and cytokine chips. The results demonstrated that PS-MNPLs induced hematopoietic toxicity, which was manifested by the disorder of bone marrow cell arrangement, the reduction in colony-forming, self-renewal and differentiation capacity, and the increased proportion of lymphocytes. PS-MNPLs also disrupted the homeostasis of the gut microbiota, metabolism, and inflammation, all of which were correlated with hematotoxicity, suggesting that abnormal gut microbiota-metabolite-cytokine axes might be the crucial pathways in MNPLs-induced hematopoietic injury. In conclusion, our study systematically demonstrated that multi-scale PS-MNPLs induced hematopoietic toxicity via the crosstalk of gut microbiota, metabolites, and cytokines and provided valuable insights into MNPLs toxicity, which was conducive to health risk assessment and informed policy decisions regarding PS-MNPLs.