T-2 toxin triggers lipid metabolism disorder and oxidative stress in liver of ducks

• Published in: Ecotoxicology and environmental safety Vol. 286; p. 117169
• Main Authors: An, Keying, Shi, Bozhi, Lv, Xueze, Liu, Yanhan, Xia, Zhaofei
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 01.11.2024; Elsevier
• Subjects: Animals; Duck; Ducks; Glutathione Peroxidase - metabolism; Hepatotoxicity; Lipid Metabolism - drug effects; Lipid Metabolism Disorders - chemically induced; Liver - drug effects; Liver - metabolism; Liver - pathology; Malondialdehyde - metabolism; Oxidative stress; Oxidative Stress - drug effects; Steatosis; T-2 toxin; T-2 Toxin - toxicity; Oxidative stress; T-2 toxin; Duck; Hepatotoxicity; Steatosis
• ISSN: 0147-6513; 1090-2414; 1090-2414
• DOI: 10.1016/j.ecoenv.2024.117169

T-2 toxin (T-2) is a highly toxic mycotoxin that threatens organism health, yet its hepatoxicity on ducks remains unknown. The present study aimed to assess the hepatoxicity and redox reactions induced by T-2 in ducks. Sixty 7-day-old ducklings were divided into 4 groups and exposed to 0, 200, 400 and 800 μg/kg bodyweight of T-2 through oral gavage for 2 weeks. The growth performance, liver histopathology, biochemical indicators, antioxidant capacity and hepatic damage-related genes of ducks were analyzed. The results revealed that 800 µg/kg T-2 inhibited the growth and feed intake of ducks, whereas liver index increased with the elevation of T-2 concentration. Histological examinations exhibited that T-2 caused hepatic cord disappeared and severe steatosis. Moreover, serum AST, ALT and TG were substantially higher in 400 μg/kg group, while γ-GT and ALB were reduced under 800 μg/kg T-2 exposure. In addition, significant increase of malondialdehyde (MDA) in liver, decrease of hepatic total antioxidant capacity (T-AOC) and serum glutathione peroxidase (GPx) were observed in all T-2 groups. Furthermore, T-2 disrupted lipid metabolism and oxidative stress-related genes expression in liver. The transcript level of fatty acid binding protein 1 (FABP1) was markedly raised in all T-2 groups, and hepatic acyl-CoA oxidase 1 (ACOX1) was significantly raised in 200 and 400 μg/kg T-2 groups. Under 800 μg/kg T-2, significant induction of hypoxia inducible factor-1 alpha (HIF-1α), and downregulated peroxisome proliferator-activated receptor (PPAR)-alpha, carnitine palmitoyl transferase 1A (CPT1A), peroxisome proliferator-activated receptor gamma coactivator 1alpha (PGC-1α), GPx1, catalase (CAT) mRNA levels were observed. Therefore, we conclude that T-2 caused liver injury through lipid metabolism disruption and oxidative stress in ducks, which reinforces understanding about the hepatoxicity mechanisms of T-2 and provides new targets for detoxication and prevention. •T-2 toxin inhibits growth performance of ducks at 800 μg/kg bodyweight.•T-2 toxin causes severe hepatic steatosis in ducks.•T-2 toxin disrupts hepatic fatty acid transportation and β-oxidation in ducks.•T-2 toxin triggers oxidative damage in ducks.