Further evidence for the existence of environmental and host-associated species of coagulase-negative staphylococci in dairy cattle

• Published in: Veterinary microbiology Vol. 172; no. 3-4; pp. 466 - 474
• Main Authors: De Visscher, Anneleen, Supré, Karlien, Haesebrouck, Freddy, Zadoks, Ruth N., Piessens, Veerle, Van Coillie, Els, Piepers, Sofie, De Vliegher, Sarne
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 27.08.2014
• Subjects: Animals; Cattle; Coagulase; Coagulase-negative Staphylococcus species; Dairy cow; Dairying; Disease Reservoirs - microbiology; Environmental Microbiology; Female; Intramammary infection; Mammary Glands, Animal - microbiology; Mastitis, Bovine - microbiology; Milk - microbiology; Parlor-associated extramammary niche; Prevalence; Species Specificity; Staphylococcal Infections - microbiology; Staphylococcal Infections - veterinary; Staphylococcus - classification; Dairy cow; Coagulase-negative Staphylococcus species; Intramammary infection; Parlor-associated extramammary niche
• ISSN: 0378-1135; 1873-2542
• DOI: 10.1016/j.vetmic.2014.06.011

•The distribution of the parlor-associated CNS species was mapped.•Each herd has its own CNS microbiota in the extramammary niches.•Staphylococcus cohnii, Staphylococcus haemolyticus and Staphylococcus sciuri can be considered opportunistic pathogens.•Staphylococcus equorum and Staphylococcus fleurettii can be considered environmental pathogens. Coagulase-negative staphylococci (CNS) are abundantly present in the dairy farm environment and on bovine skin and mucosae. They are also the most prevalent bacteria causing bovine intramammary infections (IMI). Reservoirs and transmission routes of CNS are not yet fully unraveled. The objectives of this study were to explore the distribution of CNS in parlor-related extramammary niches and to compare it to the distributions of CNS causing IMI in those herds. Niches that were targeted in this study were cows’ teat apices, milking machine unit liners, and milker's skin or gloves. Each of the three herds had its own CNS microbiota in those niches. The most prevalent species in the parlor-related extramammary niches were Staphylococcus cohnii, S. fleurettii, and S. equorum in the first, second, and third herd, respectively, whereas S. haemolyticus and S. sciuri were found in all herds. S. cohnii and S. fleurettii, as well as S. haemolyticus, which was present in each herd, were also frequently found in milk samples. By contrast, S. chromogenes, S. simulans, and S. xylosus favored the mammary gland, whereas S. equorum was more common in the parlor-associated niches. Within each herd, species distribution was similar between teat apices and milking machine unit liners. In conclusion, some of the extramammary niches related to the milking process might act as infection sources for IMI-causing CNS. This study provides further evidence that the group of CNS species is comprised of environmental, opportunistic and host-adapted species which differ in ecology.