Imaging the fetal nonhuman primate brain with SV2A positron emission tomography (PET)

• Published in: European journal of nuclear medicine and molecular imaging Vol. 49; no. 11; pp. 3679 - 3691
• Main Authors: Rossano, Samantha, Toyonaga, Takuya, Berg, Eric, Lorence, Isabella, Fowles, Krista, Nabulsi, Nabeel, Ropchan, Jim, Li, Songye, Ye, Yunpeng, Felchner, Zachary, Kukis, David, Huang, Yiyun, Benveniste, Helene, Tarantal, Alice F., Groman, Stephanie, Carson, Richard E.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.09.2022; Springer Nature B.V
• Subjects: Animals; Brain; Brain - diagnostic imaging; Brain - metabolism; Cardiology; Density; Fetuses; Glycoproteins; Imaging; In vivo methods and tests; Macaca mulatta - metabolism; Medical imaging; Medicine; Medicine & Public Health; Membrane Glycoproteins - metabolism; Monkeys; Monkeys & apes; Neonates; Nerve Tissue Proteins - metabolism; Neuroimaging; Nuclear Medicine; Oncology; Original Article; Orthopedics; Positron emission; Positron emission tomography; Positron-Emission Tomography - methods; Preclinical Imaging; Primates; Radioisotopes; Radiology; Sensorimotor integration; Synapses; Synaptic density; Synaptogenesis; Tomography; Synaptogenesis; SV2A; In utero; Positron emission tomography; Nonhuman primate
• ISSN: 1619-7070; 1619-7089; 1619-7089
• DOI: 10.1007/s00259-022-05825-6

Purpose Exploring synaptic density changes during brain growth is crucial to understanding brain development. Previous studies in nonhuman primates report a rapid increase in synapse number between the late gestational period and the early neonatal period, such that synaptic density approaches adult levels by birth. Prenatal synaptic development may have an enduring impact on postnatal brain development, but precisely how synaptic density changes in utero are unknown because current methods to quantify synaptic density are invasive and require post-mortem brain tissue. Methods We used synaptic vesicle glycoprotein 2A (SV2A) positron emission tomography (PET) radioligands [ 11 C]UCB-J and [ 18 F]Syn-VesT-1 to conduct the first assessment of synaptic density in the developing fetal brain in gravid rhesus monkeys. Eight pregnant monkeys were scanned twice during the third trimester at two imaging sites. Fetal post-mortem samples were collected near term in a subset of subjects to quantify SV2A density by Western blot. Results Image-derived fetal brain SV2A measures increased during the third trimester. SV2A concentrations were greater in subcortical regions than in cortical regions at both gestational ages. Near term, SV2A density was higher in primary motor and visual areas than respective associative regions. Post-mortem quantification of SV2A density was significantly correlated with regional SV2A PET measures. Conclusion While further study is needed to determine the exact relationship of SV2A and synaptic density, the imaging paradigm developed in the current study allows for the effective in vivo study of SV2A development in the fetal brain.