HMGB1: an important regulator of myeloid differentiation and acute myeloid leukemia as well as a promising therapeutic target

• Published in: Journal of molecular medicine (Berlin, Germany) Vol. 99; no. 1; pp. 107 - 118
• Main Authors: Liu, Lulu, Zhang, Jingjing, Zhang, Xianning, Cheng, Panpan, Liu, Lei, Huang, Qian, Liu, Haihui, Ren, Saisai, Wei, Peng, Wang, Cuiling, Dou, Cuiyun, Chen, Lulu, Liu, Xin, Zhang, Hao, Chen, Mingtai
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.01.2021; Springer Nature B.V
• Subjects: Acetic acid; Acute myeloid leukemia; Apoptosis; Biomedical and Life Sciences; Biomedicine; Bone marrow; Cell differentiation; Cell growth; Cell proliferation; High mobility group proteins; Histone deacetylase; HMGB1 protein; Human Genetics; Internal Medicine; Leukemia; Leukocytes (mononuclear); Molecular Medicine; Myeloid leukemia; Original; Original Article; Retinoic acid; Therapeutic applications; Therapeutic targets; Transforming growth factor-b; AML; HMGB1; TGFBI; Myeloid differentiation; Chidamide
• ISSN: 0946-2716; 1432-1440
• DOI: 10.1007/s00109-020-01998-5

High mobility group box 1 (HMGB1) is a non-histone nuclear protein which has been intensively studied in various physiological and pathological processes including leukemia. Here in this study, we further demonstrated that HMGB1 presents higher expression in the bone marrow mononuclear cells of acute myeloid leukemia (AML) patients compared with the normal controls and contributes to the AML pathogenesis and progression by inhibiting apoptosis, facilitating proliferation, and inducing myeloid differentiation blockade of AML cells. Mechanistic investigation revealed that transforming growth factor beta-induced (TGFBI) acts as a potential downstream target of HMGB1 and lentivirus-mediated knockdown of TGFBI expression impaired phorbol-12-myristate-13-acetate (PMA) and all-trans retinoic acid (ATRA)–induced myeloid differentiation of AML cell lines. On the other hand, chidamide, an orally histone deacetylase inhibitor, decreases HMGB1 expression significantly in AML cells with concomitant upregulation of TGFBI expression, and confers therapeutic effect on AML by inducing cell differentiation, apoptosis and inhibiting cell proliferation. In conclusion, our findings provide additional insights that HMGB1 is a promising therapeutic target of AML, and also present experimental evidence for the clinical application of chidamide as a novel agent in AML therapy by downregulating HMGB1 expression. Key messages HMGB1 induces cell proliferation and myeloid differentiation blockade and inhibits apoptosis of AML cells. TGFBI acts as a potential target of HMGB1. Chidamide, a selective HDAC inhibitor, confers promising therapeutic effect for AML via downregulating HMGB1 expression.