Epitope-Specific Antibody Response to IgE by Mimotope Immunization

• Published in: The Journal of immunology (1950) Vol. 160; no. 7; pp. 3315 - 3321
• Main Authors: Rudolf, Michael P, Vogel, Monique, Kricek, Franz, Ruf, Christine, Zurcher, Adrian W, Reuschel, Roland, Auer, Manfred, Miescher, Sylvia, Stadler, Beda M
• Format: Journal Article
• Language: English
• Published: United States Am Assoc Immnol 01.04.1998
• Subjects: Amino Acid Sequence; Animals; Antibodies, Anti-Idiotypic - biosynthesis; Antibodies, Anti-Idiotypic - chemistry; Antibodies, Anti-Idiotypic - metabolism; Antibodies, Monoclonal - chemistry; Antibodies, Monoclonal - metabolism; Antibody Specificity; Bacteriophage M13 - immunology; Binding Sites, Antibody; Binding, Competitive - immunology; CHO Cells; Clone Cells - transplantation; Cricetinae; Epitopes - administration & dosage; Epitopes - immunology; Fluoresceins - metabolism; Fluorescent Dyes; Humans; Immunoglobulin E - immunology; Immunoglobulin E - metabolism; Immunoglobulin epsilon-Chains - genetics; Molecular Sequence Data; Ouabain - analogs & derivatives; Ouabain - metabolism; Peptide Library; Peptides - administration & dosage; Peptides - immunology; Peptides - pharmacology; Protein Conformation; Rabbits; Receptors, IgG - genetics; Transfection - immunology; Vaccination
• ISSN: 0022-1767; 1550-6606
• DOI: 10.4049/jimmunol.160.7.3315

We have previously described a mouse monoclonal anti-human IgE antibody (BSW17) capable of recognizing receptor-bound IgE without inducing mediator release from human basophils or mast cells. Moreover, immune complexes of IgE and BSW17 are not able to bind to the IgE receptor. An initial attempt to map the precise epitope recognized by this mAb by using Fc epsilon-derived peptides of variable length was unsuccessful. However, by screening random peptide phage display libraries we isolated circular nona- and octapeptides specifically recognized by BSW17. These constrained peptides mimic at least a part of a conformational epitope and are thus called mimotopes. These mimotopes, either phage displayed or synthetically synthesized, did not react with any other anti-human IgE antibody tested, but efficiently inhibited the binding of human IgE to BSW17 only. The use of Rhodol-Green-labeled free cyclic peptide proved that these interactions were not carrier dependent. Immunization of rabbits with phage clones displaying the specific peptides on the surface induced an anti-human IgE response specific for the epitope of BSW17. Therefore, we conclude that such mimotopes or mimotope-derived peptides might be used for vaccination to induce in vivo a beneficial anti-IgE response as a novel immunotherapy.