Application of arteether-loaded polyurethane nanomicelles to induce immune response in breast cancer model

• Published in: Artificial cells, nanomedicine, and biotechnology Vol. 45; no. 4; pp. 808 - 816
• Main Authors: Jabbarzadegan, Mona, Rajayi, Hajar, Mofazzal Jahromi, Mirza Ali, Yeganeh, Hamid, Yousefi, Mehdi, Muhammad Hassan, Zuhair, Majidi, Jafar
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 01.06.2017; Taylor & Francis Ltd
• Subjects: 4T1; Animals; Antineoplastic Agents - chemistry; Antineoplastic Agents - pharmacology; Antineoplastic Agents - therapeutic use; Arteether; Artemisinins - chemistry; Artemisinins - pharmacology; Artemisinins - therapeutic use; Biocompatibility; Biodegradability; Breast cancer; Breast Neoplasms - drug therapy; Breast Neoplasms - immunology; Breast Neoplasms - pathology; Cancer therapy; Cell Line, Tumor; Cytokines; Cytokines - metabolism; Cytotoxicity; Drug Carriers - chemistry; Drug Liberation; Enzyme-linked immunosorbent assay; Female; Fourier transforms; Hydrogen-Ion Concentration; Hydrophobic and Hydrophilic Interactions; Immune response; Immune system; immunostimulator; Infrared radiation; Interleukin 4; Light scattering; Mammary Neoplasms, Experimental - drug therapy; Mammary Neoplasms, Experimental - immunology; Mice; Mice, Inbred BALB C; Micelles; nanocarrier; Nanoparticles - chemistry; pH effects; Photon correlation spectroscopy; Polyurethane; Polyurethane resins; Polyurethanes - chemistry; Solubility; Splenocytes; Toxicity; Tumor Burden - drug effects; Tumor Burden - immunology; Water - chemistry; γ-Interferon; 4T1; nanocarrier; Cancer therapy; immunostimulator
• ISSN: 2169-1401; 2169-141X
• DOI: 10.1080/21691401.2016.1178131

To concentrate a potent anticancer drug (Arteether) in tumor microenvironment, we encapsulated it in biodegradable and pH sensitive polyurethane (PU) nanomicelles (NMs). The nanocomplex was characterized by Fourier transform infrared (FTIR), dynamic light scattering (DLS). The loading capacity and release profile in pH of 5.4 and 7.4 were considered. The cytotoxicity effect was evaluated in vitro and in vivo settings. The level of IFN-γ and IL-4 cytokines of mice splenocytes were assessed by enzyme-linked immunosorbent assay (ELISA). The nanocomplex showed negative zeta charge of −26.2 mV, size of 42.30 nm and high loading capacity (92%). Release profile showed a faster rate of drug liberation at pH 5.4 as compared to that of pH 7.4. It indicated significant inhibitory effect on the growth of 4T1 cell line and increased IFN-γ level.