The short-term effects of RBC transfusions on intestinal injury in preterm infants

• Published in: Pediatric research Vol. 93; no. 5; pp. 1307 - 1313
• Main Authors: Kalteren, Willemien S., Bos, Arend F., Bergman, Klasien A., van Oeveren, Willem, Hulscher, Jan B. F., Kooi, Elisabeth M. W.
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.04.2023; Nature Publishing Group
• Subjects: Biomarkers; Clinical; Clinical Research Article; Enterocolitis, Necrotizing - etiology; Fatty Acid-Binding Proteins; Fatty acids; Gastrointestinal diseases; Gestational Age; Humans; Infant; Infant, Newborn; Infant, Premature; Intestinal Diseases - therapy; Intestines; Medicine; Medicine & Public Health; Necrosis; Newborn babies; Oxidative stress; Oxygen saturation; Pediatric Surgery; Pediatrics; Premature babies
• ISSN: 0031-3998; 1530-0447
• DOI: 10.1038/s41390-022-01961-9

Background Anemic preterm infants may require red blood cell (RBC) transfusions to maintain sufficient oxygen supply to vital organs. Transfusion treatment, however, may have adverse intestinal effects. We aimed to investigate the short-term effects of RBC transfusions, hypothesizing to find signs of oxidative stress and intestinal injury, possibly related to levels of splanchnic (re-)oxygenation. Methods We prospectively included preterm infants (gestational age < 32 weeks). We measured urinary biomarkers for oxidative stress (8-isoprostane) and intestinal cell injury (intestinal fatty acid-binding protein, I-FABP) shortly before and after RBC transfusion. Splanchnic oxygen saturation (r s SO 2 ) and r s SO 2 variability were assessed simultaneously. Results Twenty-nine preterm infants received 58 RBC transfusions at various postnatal ages. Six of them developed necrotizing enterocolitis (NEC) after transfusion. Urinary 8-isoprostane and I-FABP increased following RBC transfusion (median 282–606 pg/ml and 4732–6968 pg/ml, p < 0.01), more pronounced in infants who developed NEC. Change in I-FABP correlated with change in 8-isoprostane (rho = 0.623, p  < 0.01). Lower r s SO 2 variability, but not higher mean r s SO 2 was associated with higher 8-isoprostane and I-FABP levels after transfusion. Conclusions Preterm RBC transfusions are associated with concomitant signs of oxidative stress and intestinal injury, parallel with lower variability in splanchnic oxygenation. This may represent the early pathogenetic process of transfusion-associated NEC. Impact Red blood cell (RBC) transfusions in preterm infants are associated with a near 2-fold increase in urinary biomarkers for oxidative stress (8-isoprostane) and intestinal cell injury (intestinal fatty acid-binding protein, I-FABP). Magnitude of change in I-FABP strongly correlated with the magnitude of 8-isoprostane change, suggesting a role for oxidative stress in the pathogenesis of intestinal injury. Lower splanchnic oxygen saturation variability following RBC transfusion was associated with higher 8-isoprostane and I-FABP levels. Loss of splanchnic variability after RBC transfusion may result from increased oxidative stress and its concomitant intestinal injury, possibly representing the early pathogenetic process of transfusion-associated necrotizing enterocolitis.