Protective effect of astrocyte-conditioned medium on neurons following hypoxia and mechanical injury

• Published in: Chinese journal of traumatology Vol. 16; no. 1; pp. 3 - 9
• Main Authors: Yan, Ji-wen, Tan, Tong-yan, Huang, Qi-lin
• Format: Journal Article
• Language: English
• Published: China Elsevier B.V 01.02.2013; Department of Surgery, CAPF 8710 Hospital, Putian City 351100, Fujian Province, China%Medical Team of 95100 Troops, Guangzhou 510405,China%Department of Neurosurgery, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China; Elsevier
• Subjects: Animals; Astrocytes - physiology; ATP酶活性; Cell Hypoxia; Cell Survival; Cells, Cultured; Culture Media, Conditioned; Glial cell line-derived neurotrophic factor; Mice; Nerve Growth Factors - physiology; Neurons; Neurons - physiology; 乳酸脱氢酶; 保护作用; 星形胶质细胞; 机械性损伤; 条件培养液; 神经元; 缺氧复氧; Neurons; Glial cell line-derived neurotrophic factor; Cell hypoxia; Gliai cell line-derived neurotrophic factor
• ISSN: 1008-1275
• DOI: 10.3760/cma.j.issn.1008-1275.2013.01.001

Objective： To investigate the protective effect of mouse astrocyte-conditioned medium （ACM） on hypoxic and mechanically injured neurons by a cell model in vitro, and to explore the possible mechanism. Methods： The model of hypoxic neuronal injury was caused by 3% 02 in three-gas incubator. Neurons were cultured with ordinary medium or 20% ACM respectively and randomly divided into hypoxic group （hypoxia for 4, 8, 24 h and marked as H4R0, H8R0, H24R0） and hypoxia reoxygenation group （H4R24, H8R24, H24R24）. Mechanical injury model was developed by scratching neurons cultured in 20% ACM or ordinary medium to different degrees. Neu- rons in both medium were divided into normal control group, mild, moderate and severe injury groups. The 20% ACM was added 24 h before hypoxia/reoxygenation or mechanical injury. The morphology and survival of neurons were observed and counted by trypan blue staining. The concentration of NO, lactic dehydrogenase （LDH） and membrane ATPase activity were detected by corresponding kits. Results： It was showed that 20% ACM can obviously promote the survival rate of hypoxia/reoxygenated neurons and scratched neurons as well. The morphology and num- ber of neurons exposed to hypoxia or scratch injury showed great difference between groups with or without ACM treatment. Compared with control group, the concentration of NO and LDH was much lower in hypoxic/reoxygenated neurons treated with 20% ACM, and the ATPase activity was higher. For the mechanical injury model, neurons with moderate injury also revealed a lower NO and LDH concen- tration than the control group. All the differences were sta- tistically significant （P〈0.05）. Conclusion： ACM can promote the survival and func- tional recovery of neurons following hypoxia or scratching to a certain degree. The mechanism may be associated with reducing the synthesis and release of NO and LDH as well as increasing the activity of membrane ATPase.