Photoacoustic stimulation promotes the osteogenic differentiation of bone mesenchymal stem cells to enhance the repair of bone defect

• Published in: Scientific reports Vol. 7; no. 1; pp. 15842 - 14
• Main Authors: Huang, Zebin, Xu, Jiankun, Chen, Jiebin, Chen, Hongjiang, Wang, Hailong, Huang, Zhonglian, Chen, Youbin, Lu, Xiaolin, Lu, Fushen, Hu, Jun
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 20.11.2017; Nature Publishing Group
• Subjects: 13/100; 13/107; 13/51; 631/532/2074; 631/80/641; Alkaline phosphatase; Alkaline Phosphatase - genetics; Animals; Bone Development - drug effects; Bone marrow; Bone Marrow Cells - drug effects; Bone Marrow Cells - metabolism; Bone Regeneration; Calcium; Calcium - metabolism; Cell Differentiation - drug effects; Defects; Femur; Femur - drug effects; Femur - growth & development; Glycolic acid; Graphite - chemistry; Humanities and Social Sciences; Male; Mesenchymal stem cells; Mesenchymal Stem Cells - drug effects; Mesenchymal Stem Cells - metabolism; Mesenchyme; multidisciplinary; Osteogenesis; Osteogenesis - drug effects; Osteogenesis - genetics; Osteopontin; Osteopontin - genetics; Polylactic Acid-Polyglycolic Acid Copolymer - administration & dosage; Polylactic Acid-Polyglycolic Acid Copolymer - chemistry; Polylactide-co-glycolide; Rats; Rats, Sprague-Dawley; Regeneration; Science; Science (multidisciplinary); Skin & tissue grafts; Stem cell transplantation; Stem cells; Tissue Engineering; Tissue Scaffolds
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-017-15879-4

The aim of this study was to evaluate the direct photoacoustic (PA) effect on bone marrow mesenchymal stem cells (BMSCs) which is a key cell source for osteogenesis. As scaffold is also an indispensable element for tissue regeneration, here we firstly fabricated a composited sheet using polylactic-co-glycolic acid (PLGA) mixing with graphene oxide (GO). BMSCs were seeded on the PLGA-GO sheets and received PA treatment in vitro for 3, 9 and 15 days, respectively. Then the BMSCs were harvested and subjected to assess alkaline phosphatase (ALP) activity, calcium content and osteopontin (OPN) on 3, 9 and 15 days. For in vivo study, PLGA-GO sheet seeded with BMSCs after in vitro PA stimulation for 9 days were implanted to repair the bone defect established in the femoral mid-shaft of Sprague-Dawley rat. PLGA-GO group with PA pretreatment showed promising outcomes in terms of the expression of ALP, OPN, and calcium content, thus enhanced the repair of bone defect. In conclusion, we have developed an alternative approach to enhance the repair of bone defect by making good use of the beneficial effect of PA.