Benzene containing polyhydroxyalkanoates homo- and copolymers synthesized by genome edited Pseudomonas entomophila

Microbial synthesis of functional polymers has become increasingly important for industrial biotechnology. For the first time, it became possible to synthesize controllable composition of poly（3-hydroxyalkanoate） （P3HA） consisting of 3-hydroxydodec- anoate （3HDD） and phenyl group on the side-chain w...

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Published inScience China. Life sciences Vol. 57; no. 1; pp. 4 - 10
Main Authors Shen, Rui, Cai, LongWei, Meng, DeChuan, Wu, LinPing, Guo, Kai, Dong, GuoXing, Liu, Lei, Chen, JinChun, Wu, Qiong, Chen, GuoQiang
Format Journal Article
LanguageEnglish
Published Beijing Science China Press 2014
Springer Nature B.V
Subjects
Benzene - analysis
Biomedical and Life Sciences
Chromosomes, Bacterial
Culture Media
Genome, Bacterial
Life Sciences
Nuclear Magnetic Resonance, Biomolecular
Polyhydroxyalkanoates - chemistry
Polyhydroxyalkanoates - metabolism
Pseudomonas
Pseudomonas - genetics
Pseudomonas - metabolism
Research Paper
假单胞菌
均聚物
基因组
微生物合成
无规共聚物
编辑
聚羟基脂肪酸酯
苯基
oxidation
synthetic biology
polyhydroxyalkanoates
PHB
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Summary:Microbial synthesis of functional polymers has become increasingly important for industrial biotechnology. For the first time, it became possible to synthesize controllable composition of poly（3-hydroxyalkanoate） （P3HA） consisting of 3-hydroxydodec- anoate （3HDD） and phenyl group on the side-chain when chromosome of Pseudomonas entomophila was edited to weaken its t-oxidation. Cultured in the presence of 5-phenylvaleric acid （PVA）, the edited P. entomophila produced only homopolymer poly（3-hydroxy-5-phenylvalerate） or P（3HPhV）. While copolyesters P（3HPhV-co-3HDD） of 3-hydroxy-5-phenylvalerate （3HPhV） and 3-hydroxydodecanoate （3HDD） were synthesized when the strain was grown on mixtures of PVA and dodecanoic acid （DDA）. Compositions of 3HPhV in P（3HPhV-co-3HDD） were controllable ranging from 3% to 32% depending on DDDA/PVA ratios. Nuclear magnetic resonance （NMR） spectra clearly indicated that the polymers were homopolymer of P（3HPhV） and random copolymers of 3HPhV and 3HDD. Their mechanical and thermal properties varied dramatically de- pending on the monomer ratios. Our results demonstrated the possibility to produce tailor-made, novel functional PHA using the chromosome edited P. entomophila.
Bibliography:polyhydroxyalkanoates, PHB, Pseudomonas entomophila, β-oxidation, synthetic biology
Microbial synthesis of functional polymers has become increasingly important for industrial biotechnology. For the first time, it became possible to synthesize controllable composition of poly（3-hydroxyalkanoate） （P3HA） consisting of 3-hydroxydodec- anoate （3HDD） and phenyl group on the side-chain when chromosome of Pseudomonas entomophila was edited to weaken its t-oxidation. Cultured in the presence of 5-phenylvaleric acid （PVA）, the edited P. entomophila produced only homopolymer poly（3-hydroxy-5-phenylvalerate） or P（3HPhV）. While copolyesters P（3HPhV-co-3HDD） of 3-hydroxy-5-phenylvalerate （3HPhV） and 3-hydroxydodecanoate （3HDD） were synthesized when the strain was grown on mixtures of PVA and dodecanoic acid （DDA）. Compositions of 3HPhV in P（3HPhV-co-3HDD） were controllable ranging from 3% to 32% depending on DDDA/PVA ratios. Nuclear magnetic resonance （NMR） spectra clearly indicated that the polymers were homopolymer of P（3HPhV） and random copolymers of 3HPhV and 3HDD. Their mechanical and thermal properties varied dramatically de- pending on the monomer ratios. Our results demonstrated the possibility to produce tailor-made, novel functional PHA using the chromosome edited P. entomophila.
11-5841/Q
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1674-7305
1869-1889
DOI:10.1007/s11427-013-4596-8