Photochemical Control of the Infectivity of Adenoviral Vectors Using a Novel Photocleavable Biotinylation Reagent

We have explored a novel strategy for controlling the infectivity of adenoviral vectors. This strategy involves a method whereby the infectivity of adenoviral vectors is neutralized by treatment of viral particles with a water-soluble, photocleavable biotinylation reagent. These modified viral vecto...

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Published inChemistry & biology Vol. 9; no. 5; pp. 567 - 573
Main Authors Pandori, Mark W, Hobson, David A, Olejnik, Jerzy, Krzymanska-Olejnik, Edyta, Rothschild, Kenneth J, Palmer, Abraham A, Phillips, Tamara J, Sano, Takeshi
Format Journal Article
LanguageEnglish
Published United States Elsevier Ltd 01.05.2002
Subjects
Adenoviridae - drug effects
Adenoviridae - pathogenicity
Adenoviridae - radiation effects
Animals
Biotin - chemical synthesis
Biotin - chemistry
Biotinylation - methods
Blotting, Western - methods
Cell Survival - radiation effects
Dogs
Gene Expression - genetics
Genetic Vectors - drug effects
Genetic Vectors - radiation effects
Humans
Mice
Mice, Nude
Photochemistry - methods
Time Factors
Tumor Cells, Cultured
Ultraviolet Rays
Virulence - radiation effects
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Abstract We have explored a novel strategy for controlling the infectivity of adenoviral vectors. This strategy involves a method whereby the infectivity of adenoviral vectors is neutralized by treatment of viral particles with a water-soluble, photocleavable biotinylation reagent. These modified viral vectors possess little to no infectivity for target cells. Exposure of these modified viral vectors to 365 nm light induces a reversal of the neutralizing, chemical modification, resulting in restoration of infectivity to the viral vectors. The light-directed transduction of target cells by photoactivatable adenoviral vectors was demonstrated successfully both in vitro and in vivo. This photochemical infectivity trigger possesses great potential, both as a research tool and as a novel tactic for the delivery of gene-transfer agents, since the infectivity of adenoviral vectors can be controlled externally in a versatile manner.
AbstractList We have explored a novel strategy for controlling the infectivity of adenoviral vectors. This strategy involves a method whereby the infectivity of adenoviral vectors is neutralized by treatment of viral particles with a water-soluble, photocleavable biotinylation reagent. These modified viral vectors possess little to no infectivity for target cells. Exposure of these modified viral vectors to 365 nm light induces a reversal of the neutralizing, chemical modification, resulting in restoration of infectivity to the viral vectors. The light-directed transduction of target cells by photoactivatable adenoviral vectors was demonstrated successfully both in vitro and in vivo. This photochemical infectivity trigger possesses great potential, both as a research tool and as a novel tactic for the delivery of gene-transfer agents, since the infectivity of adenoviral vectors can be controlled externally in a versatile manner.
Author Pandori, Mark W
Phillips, Tamara J
Krzymanska-Olejnik, Edyta
Rothschild, Kenneth J
Olejnik, Jerzy
Palmer, Abraham A
Hobson, David A
Sano, Takeshi
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10.1073/pnas.92.16.7590
10.1038/sj.gt.3301338
10.1038/11400
10.1016/0003-2697(87)90587-2
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SubjectTerms Adenoviridae - drug effects
Adenoviridae - pathogenicity
Adenoviridae - radiation effects
Animals
Biotin - chemical synthesis
Biotin - chemistry
Biotinylation - methods
Blotting, Western - methods
Cell Survival - radiation effects
Dogs
Gene Expression - genetics
Genetic Vectors - drug effects
Genetic Vectors - radiation effects
Humans
Mice
Mice, Nude
Photochemistry - methods
Time Factors
Tumor Cells, Cultured
Ultraviolet Rays
Virulence - radiation effects
Title Photochemical Control of the Infectivity of Adenoviral Vectors Using a Novel Photocleavable Biotinylation Reagent
URI https://dx.doi.org/10.1016/S1074-5521(02)00135-7
https://www.ncbi.nlm.nih.gov/pubmed/12031663
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