Photochemical Control of the Infectivity of Adenoviral Vectors Using a Novel Photocleavable Biotinylation Reagent

• Published in: Chemistry & biology Vol. 9; no. 5; pp. 567 - 573
• Main Authors: Pandori, Mark W, Hobson, David A, Olejnik, Jerzy, Krzymanska-Olejnik, Edyta, Rothschild, Kenneth J, Palmer, Abraham A, Phillips, Tamara J, Sano, Takeshi
• Format: Journal Article
• Language: English
• Published: United States Elsevier Ltd 01.05.2002
• Subjects: Adenoviridae - drug effects; Adenoviridae - pathogenicity; Adenoviridae - radiation effects; Animals; Biotin - chemical synthesis; Biotin - chemistry; Biotinylation - methods; Blotting, Western - methods; Cell Survival - radiation effects; Dogs; Gene Expression - genetics; Genetic Vectors - drug effects; Genetic Vectors - radiation effects; Humans; Mice; Mice, Nude; Photochemistry - methods; Time Factors; Tumor Cells, Cultured; Ultraviolet Rays; Virulence - radiation effects
• ISSN: 1074-5521; 1879-1301
• DOI: 10.1016/S1074-5521(02)00135-7

We have explored a novel strategy for controlling the infectivity of adenoviral vectors. This strategy involves a method whereby the infectivity of adenoviral vectors is neutralized by treatment of viral particles with a water-soluble, photocleavable biotinylation reagent. These modified viral vectors possess little to no infectivity for target cells. Exposure of these modified viral vectors to 365 nm light induces a reversal of the neutralizing, chemical modification, resulting in restoration of infectivity to the viral vectors. The light-directed transduction of target cells by photoactivatable adenoviral vectors was demonstrated successfully both in vitro and in vivo. This photochemical infectivity trigger possesses great potential, both as a research tool and as a novel tactic for the delivery of gene-transfer agents, since the infectivity of adenoviral vectors can be controlled externally in a versatile manner.