PI(3,4,5)P3 regulates the interaction between Akt and B23 in the nucleus
Phosphatidylinositol (3,4,5)-triphosphate (PIP₃) is a lipid second messenger that employs a wide range of downstream effector proteins for the regulation of cellular processes, including cell survival, polarization and proliferation. One of the most well characterized cytoplasmic targets of PIP₃, se...
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Published in | BMB reports Vol. 43; no. 2; pp. 127 - 132 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Korea (South)
생화학분자생물학회
01.02.2010
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Subjects | |
Online Access | Get full text |
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Summary: | Phosphatidylinositol (3,4,5)-triphosphate (PIP₃) is a lipid second messenger that employs a wide range of downstream effector proteins for the regulation of cellular processes, including cell survival, polarization and proliferation. One of the most well characterized cytoplasmic targets of PIP₃, serine/threonine protein kinase B (PKB)/Akt, promotes cell survival by directly interacting with nucleophosmin (NPM)/B23, the nuclear target of PIP₃. Here, we report that nuclear PIP₃ competes with Akt to preferentially bind B23 in the nucleoplasm. Mutation of Arg23 and Arg25 in the PH domain of Akt prevents binding to PIP₃, but does not disrupt the Akt/B23 interaction. However, treatment with phosphatases PTEN or SHIP abrogates the association between Akt and B23, indicating that nuclear PIP₃ regulates the Akt/B23 interaction by controlling the concentration and subcellular dynamics of these two proteins. |
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Bibliography: | A50 2011000020 G704-SER000001672.2010.43.2.010 |
ISSN: | 1976-6696 1976-670X |
DOI: | 10.5483/bmbrep.2010.43.2.127 |