Paroxetine induced larva zebrafish cardiotoxicity through inflammation response

• Published in: Ecotoxicology and environmental safety Vol. 260; p. 115096
• Main Authors: Zhu, Yuanhui, Song, Feifei, Gu, Jie, Wu, Linlin, Wu, Wenzhu, Ji, Guixiang
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 15.07.2023; Elsevier
• Subjects: Cardiotoxicity; Inflammation response; Larva zebrafish; Paroxetine; Paroxetine; hand2; vmhc; Larva zebrafish; amhc; Cardiotoxicity; IL-1β; IL-8; ta; IL-10; nkx2.5; TNF-α; tbx20; tbx6; tbx16; Inflammation response
• ISSN: 0147-6513; 1090-2414
• DOI: 10.1016/j.ecoenv.2023.115096

Paroxetine (PRX) is a common antidepressant drug which widely existence in natural environment. Numerous studies in the past few decades have focused on the beneficial effects of PRX on depression, however, the toxic properties and the potential mechanisms remain unclear. In this study, zebrafish embryos were exposed to 1.0, 5.0, 10 and 20 mg/L of PRX from 4 to 120-hour-post-fertilization (hpf), and it showed that PRX exposure caused adverse effects in zebrafish embryos, including decreased body length, blood flow velocity, cardiac frequency, cardiac output and increased burst activity and atria area. Meanwhile, the Tg (myl7: EGFP) and Tg (lyz: DsRed) transgenic zebrafish were used to detect the cardiotoxicity and inflammation response of PRX. Moreover, the heart development associated genes (vmhc, amhc, hand2, nkx2.5, ta, tbx6, tbx16 and tbx20) and inflammatory genes (IL-10, IL-1β, IL-8 and TNF-α) were up-regulated after PRX challenge. In addition, Aspirin was used to alleviate the PRX-induced heart development disorder. In conclusion, our study verified the PRX induced inflammatory related cardiotoxicity in larva zebrafish. Meanwhile, the current study shown the toxic effects of PRX in aquatic organism, and provide for the environmental safety of PRX. [Display omitted] •Paroxetine (PRX) is widely existence in aquatic environment.•PRX induced the cardiac toxic effect in larva zebrafish.•Inflammatory and cardiac developmental related genes were increased by PRX.•Inflammation response is the potential mechanism of PRX induced cardiac disorder.