Identification and characterization of jasmonic acid- and linolenic acid-mediated transcriptional regulation of secondary laticifer differentiation in Hevea brasiliensis

• Published in: Scientific reports Vol. 9; no. 1; pp. 14296 - 16
• Main Authors: Loh, Swee Cheng, Othman, Ahmad Sofiman, Veera Singham, G.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 04.10.2019; Nature Publishing Group
• Subjects: 14/63; 45; 45/22; 45/90; 45/91; 631/449/1659; 631/449/1736; 631/449/2653; Acids; alpha-Linolenic Acid - chemistry; Antisense RNA; Bark; Cyclopentanes - chemistry; Gene Expression Regulation, Plant; Gene families; Gene regulation; Genes, Plant; Helix-loop-helix proteins (basic); Hevea - genetics; Hevea - metabolism; Hevea brasiliensis; Homeobox; Humanities and Social Sciences; Jasmonic acid; Latex; Latex - metabolism; Linolenic acid; multidisciplinary; Organophosphorus Compounds - chemistry; Oxylipins - chemistry; Plant Proteins - genetics; Plant Proteins - metabolism; Ribonucleic acid; RNA; Science; Science (multidisciplinary); Transcription factors; Transcription Factors - genetics; Transcription Factors - metabolism
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-019-50800-1

Hevea brasiliensis remains the primary crop commercially exploited to obtain latex, which is produced from the articulated secondary laticifer. Here, we described the transcriptional events related to jasmonic acid (JA)- and linolenic acid (LA)-induced secondary laticifer differentiation (SLD) in H. brasiliensis clone RRIM 600 based on RNA-seq approach. Histochemical approach proved that JA- and LA-treated samples resulted in SLD in H. brasiliensis when compared to ethephon and untreated control. RNA-seq data resulted in 86,614 unigenes, of which 2,664 genes were differentially expressed in JA and LA-induced secondary laticifer harvested from H. brasiliensis bark samples. Among these, 450 genes were unique to JA and LA as they were not differentially expressed in ethephon-treated samples compared with the untreated samples. Most transcription factors from the JA- and LA-specific dataset were classified under MYB, APETALA2/ethylene response factor (AP2/ERF), and basic-helix-loop-helix (bHLH) gene families that were involved in tissue developmental pathways, and we proposed that Bel5-GA2 oxidase 1-KNOTTED-like homeobox complex are likely involved in JA- and LA-induced SLD in H. brasiliensis . We also discovered alternative spliced transcripts, putative novel transcripts, and cis -natural antisense transcript pairs related to SLD event. This study has advanced understanding on the transcriptional regulatory network of SLD in H. brasiliensis .