Seven-day Green Tea Supplementation Revamps Gut Microbiome and Caecum/Skin Metabolome in Mice from Stress

• Published in: Scientific reports Vol. 9; no. 1; pp. 18418 - 11
• Main Authors: Jung, Eun Sung, Park, Jong il, Park, Hyunjoon, Holzapfel, Wilhelm, Hwang, Jae Sung, Lee, Choong Hwan
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 05.12.2019; Nature Publishing Group
• Subjects: 38/77; 631/92/320; 631/92/613; 64/60; Amino acids; Amino Acids - metabolism; Animals; Bifidobacterium - drug effects; Bifidobacterium - growth & development; Bifidobacterium - isolation & purification; Caffeine; Caffeine - isolation & purification; Caffeine - pharmacology; Catechin - analogs & derivatives; Catechin - isolation & purification; Catechin - pharmacology; Cecum - drug effects; Cecum - microbiology; Cecum - radiation effects; Clostridium butyricum - drug effects; Clostridium butyricum - growth & development; Clostridium butyricum - isolation & purification; Dietary supplements; Digestive system; Epigallocatechin gallate; Fatty acids; Fatty Acids - metabolism; Female; Gastrointestinal tract; Glutamates - isolation & purification; Glutamates - pharmacology; Green tea; Humanities and Social Sciences; Intestinal microflora; Intestine; Lactobacillus; Lactobacillus - drug effects; Lactobacillus - growth & development; Lactobacillus - isolation & purification; Metabolites; Metabolome - physiology; Metabolomics; Mice; Microbiomes; Microbiota; Microbiota - drug effects; multidisciplinary; Plant Extracts - pharmacology; Prebiotics - analysis; Science; Science (multidisciplinary); Short term; Skin; Skin - drug effects; Skin - microbiology; Skin - radiation effects; Stress response; Stress, Physiological - drug effects; Tea; Tea - chemistry; Theanine; Ultraviolet Rays
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-019-54808-5

Green tea supplementation has beneficial health effects. However, its underlying mechanisms, such as effects on modulating the intestinal microbiome and endogenous metabolome, particularly following short-term supplementation, are largely unclear. We conducted an integrative metabolomics study to evaluate the effects of short-term (7-day) supplementation of green tea extract (GTE) or its components, epigallocatechin gallate, caffeine, and theanine, on the caecum microbiota and caecum/skin metabolome in mice. Further, we established an integrative metabolome-microbiome model for correlating gut and skin findings. The effects of short-term supplementation with dietary compounds were evaluated with respect to UV stress response, with GTE showing the most remarkable effects. Biplot analysis revealed that Bifidobacteria and Lactobacillus spp. were considerably influenced by short-term GTE supplementation, while Clostridium butyricum was significantly increased by UV stress without supplementation. GTE supplementation helped the skin metabolome defend against UV stress. Interestingly, a significant positive correlation was observed between caecum bacteria (Bifidobacteria, Lactobacillus spp.) and metabolites including skin barrier function-related skin metabolites, caecal fatty acids, and caecal amino acids. Overall, 7-day GTE supplementation was sufficient to alter the gut microbiota and endogenous caecum/skin metabolome, with positive effects on UV stress response, providing insight into the mechanism of the prebiotic effects of GTE supplementation.