The efficacy of a paeoniflorin-sodium alginate-gelatin skin scaffold for the treatment of diabetic wound: An in vivo study in a rat model

• Published in: Biomedicine & pharmacotherapy Vol. 151; p. 113165
• Main Authors: Yu, Haiyang, Gong, Wen, Mei, Junhao, Qin, Lihao, Piao, Zeyu, You, Deshu, Gu, Wenxian, Jia, Zhongzhi
• Format: Journal Article
• Language: English
• Published: France Elsevier Masson SAS 01.07.2022; Elsevier
• Subjects: 3D bio-printing; Diabetes; Paeoniflorin; Skin scaffold; Wound; Skin scaffold; Paeoniflorin; 3D bio-printing; Diabetes; Wound; SA
• ISSN: 0753-3322; 1950-6007
• DOI: 10.1016/j.biopha.2022.113165

To investigate the efficacy of a paeoniflorin-sodium alginate (SA)-gelatin skin scaffold for treating diabetic wound in a rat model. Bioinks were prepared using various percentages of paeoniflorin in the total weight of a solution containing SA and gelatin. Skin scaffolds containing 0%, 1%, 3%, 5%, and 10% paeoniflorin were printed using 3D bioprinting technology, and scaffold microstructure was observed with scanning electron microscopy. Skin scaffolds were then used in rats with diabetic wounds. H&E staining, Masson staining, and immunohistochemical staining for IL-1β and CD31 were performed on days 7 and 14. All skin scaffolds had a mesh-like structure with uniform pore distribution. Wounds healed well in each group, with the 1% and 3% groups demonstrating the most complete healing. H&E staining showed that skin accessory organs had appeared in each group. On day 7, collagen deposition in the 3% group was higher than in the other groups (P＜0.05), and IL-1β infiltration was lower in the 10% group than in the 3% group (P = 0.002). On day 14, IL-1β infiltration was not significantly different between the 10% and 3% groups (P = 0.078). The CD31 level was higher in the 3% group than in the other groups on days 7 and 14 (P＜0.05). A 3% paeoniflorin-SA-gelatin skin scaffold promoted the healing of diabetic wounds in rats. This scaffold promoted collagen deposition and microvascular regeneration and demonstrated anti-inflammatory properties, suggesting that this scaffold type could be used to treat diabetic wounds. [Display omitted] •3D bio-printing.•The effect of paeoniflorin.•Promoting diabetic wound healing.