Interaction between probiotic additive and perfluorobutanesulfonate pollutant on offspring growth and health after parental exposure using zebrafish

• Published in: Ecotoxicology and environmental safety Vol. 214; p. 112107
• Main Authors: Liu, Mengyuan, Tang, Lizhu, Hu, Chenyan, Sun, Baili, Huang, Zileng, Chen, Lianguo
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 01.05.2021; Elsevier
• Subjects: Animals; Antagonistic interaction; Energy Metabolism - drug effects; Female; Fluorocarbons - toxicity; Hypothalamo-Hypophyseal System; Lactobacillus rhamnosus; Larva - drug effects; Larva - growth & development; Male; Offspring growth; Parental exposure; PFBS; Probiotic; Probiotics - pharmacology; Proteomics; Sulfonic Acids - toxicity; Water Pollutants, Chemical - toxicity; Zebrafish; Zebrafish - growth & development; Probiotic; Zebrafish; PFBS; Parental exposure; Antagonistic interaction; Offspring growth
• ISSN: 0147-6513; 1090-2414
• DOI: 10.1016/j.ecoenv.2021.112107

Perfluorobutanesulfonate (PFBS) pollutant and probiotic bacteria can interact to affect the reproductive outcomes of zebrafish. However, it is still unexplored how the growth and health of offspring are modulated by the combination of PFBS and probiotic. In the present study, adult zebrafish were exposed to 0 and 10 μg/L PFBS for 40 days, with or without dietary supplementation of probiotic Lactobacillus rhamnosus. After parental exposure, the development, growth and viability of offspring larvae were examined, with the integration of molecular clues across proteome fingerprint, growth hormone/insulin-like growth factor (GH/IGF) axis, calcium homeostasis, hypothalamic-pituitary-adrenal (HPA) axis and nutrient metabolism. Parental probiotic supplementation significantly increased the body weight and body length of offspring larvae. Despite the spiking of PFBS, larvae from the combined exposure group still had longer body length. RNA processing and ribosomal assembly pathways may underlie the enhancement of offspring growth by probiotic bacteria. However, the presence of PFBS remarkably increased the concentrations of cortisol hormone in offspring larvae as means to cope with the xenobiotic stress, which required more energy production. As evidenced by the proteomic analysis, the addition of probiotic bacteria likely alleviated the energy metabolism disorders of PFBS, thus allocating more energy for the larval offspring growth from the combined group. It was noteworthy that multiple molecular disturbances caused by PFBS were antagonized by probiotic additive. Overall, the present study elucidated the intergenerational interaction between PFBS and probiotic on offspring growth and health after parental exposure. [Display omitted] •Parental probiotic intake promoted the growth of offspring zebrafish larvae.•PFBS and probiotic coexposure led to the uncoupling of body weight and body length.•RNA splicing and ribosomal assembly were related to the enhanced offspring growth.•PFBS spiking depleted the energy production to cope with stress.•Antagonistic interaction between PFBS and probiotic was noted in offspring.