Quantum dot–NanoLuc bioluminescence resonance energy transfer enables tumor imaging and lymph node mapping in vivo

A small luciferase protein (Nluc) was conjugated to QDs as a bioluminescence resonance energy transfer (BRET) pair. The conjugate showed 76% BRET efficiency and lymph node mapping was successfully performed. The cRGD peptide was conjugated to QD-Nluc for tumor targeting. The self-illuminating QD-Nlu...

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Published inChemical communications (Cambridge, England) Vol. 52; no. 43; pp. 6997 - 7000
Main Authors Kamkaew, Anyanee, Sun, Haiyan, England, Christopher G., Cheng, Liang, Liu, Zhuang, Cai, Weibo
Format Journal Article
LanguageEnglish
Published England 19.05.2016
Subjects
Animals
Bioluminescence
Bioluminescence Resonance Energy Transfer Techniques - methods
Brain Neoplasms - diagnostic imaging
Cell Line, Tumor
chemical compounds
chemical reactions
Conjugates
Contrast Media - chemical synthesis
Contrast Media - chemistry
Decapoda (Crustacea)
Energy transfer
Fluorescent Dyes - chemistry
Glioblastoma - diagnostic imaging
Humans
image analysis
luciferase
Luciferases - chemistry
Luminescence
Lymph
lymph nodes
Lymph Nodes - diagnostic imaging
Mapping
Mice
Microscopy, Confocal
neoplasms
Optimization
peptides
Peptides, Cyclic - chemistry
Proteins
Quantum Dots - chemistry
Qunatum dots
Tumors
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Summary:A small luciferase protein (Nluc) was conjugated to QDs as a bioluminescence resonance energy transfer (BRET) pair. The conjugate showed 76% BRET efficiency and lymph node mapping was successfully performed. The cRGD peptide was conjugated to QD-Nluc for tumor targeting. The self-illuminating QD-Nluc showed excellent energy transfer in a living system and offered an optimal tumor-to-background ratio (>85).
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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These authors contributed equally to this work
ISSN:1359-7345
1364-548X
1364-548X
DOI:10.1039/C6CC02764D