Transcriptional, secondary metabolic, and antioxidative investigations elucidate the rapid response mechanism of Pontederia cordata to cadmium

Pontederia cordata is previously demonstrated a cadmium (Cd) tolerant plant, and also a candidate for the phytoremediation of heavy-metal-contaminated wetlands. A hydroponic experiment was used to investigate variations in photosynthetic gas exchange parameters, antioxidative activities, chlorophyll...

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Published inEcotoxicology and environmental safety Vol. 232; p. 113236
Main Authors Xin, Jianpan, Zhao, Chu, Li, Yan, Ma, Sisi, Tian, Runan
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier Inc 01.03.2022
Elsevier
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Summary:Pontederia cordata is previously demonstrated a cadmium (Cd) tolerant plant, and also a candidate for the phytoremediation of heavy-metal-contaminated wetlands. A hydroponic experiment was used to investigate variations in photosynthetic gas exchange parameters, antioxidative activities, chlorophyll and secondary metabolite contents, and transcriptome in leaves of the plant exposed to 0.44 mM Cd2+ for 0 h, 24 h, and 48 h. Under Cd2+ exposure for 24 h, the plant presented a favorable photosynthesis by maintaining relatively higher antioxidant activity. Cd2+ exposure for 48 h accelerated membrane peroxidation, declined photosynthetic pigment content, and increased polyphenol oxidase activity, thus interfering with photosynthesis. The phenylpropane pathway served as a chemical rather than physical defense against Cd2+ in the plant leaves. A total of 20,998, 4743, and 4413 differentially expressed genes (DEGs) were identified in the groups of 0 h vs 24 h, 0 h vs 48 h, and 24 h vs 48 h, respectively. The primary metabolic pathways of the DEGs were mainly enriched in nitrogen metabolism, starch and sucrose metabolism, fructose and mannose metabolism, as well as pentose-phosphate pathway, contributing to a stable cell structure and function. Flavonoid biosynthesis directly or indirectly played an antioxidative role against Cd2+ in the leaves. Forty-nine transcription factor (TF) families were identified, and 8 TF families were shared among the three groups. The present study provides a theoretical foundation for investigating tolerance mechanisms of wetland plants to Cd stress in terms of secondary metabolism and transcriptional regulation. •Phenylpropane pathway functions as a chemical defense in the plant against Cd.•Transcriptomic on mechanisms underlying Cd-tolerance in the plant was first reported.•SBP and bHLH related to flavones biosynthesis accounted for the plant Cd tolerance.•Declined physical defense of the plant to Cd was attributed to downregulated POD and CAD.
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ISSN:0147-6513
1090-2414
DOI:10.1016/j.ecoenv.2022.113236