Ammonia exposure causes the imbalance of the gut-brain axis by altering gene networks associated with oxidative metabolism, inflammation and apoptosis

• Published in: Ecotoxicology and environmental safety Vol. 224; p. 112668
• Main Authors: Li, Yutao, Pan, Lei, Zeng, Xiangyin, Zhang, Runxiang, Li, Xiang, Li, Jianhong, Xing, Houjuan, Bao, Jun
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 01.11.2021; Elsevier
• Subjects: Ammonia; Brain-gut peptide; Gut-brain axis; Pig; Toxicity assessment; BLK; ERO1A; PFKFB1; EPHA7; Toxicity assessment; Gut-brain axis; DIO1; ISG15; CD3G; TREM2; CYP3A39; OASL; Pig; Ammonia; Brain-gut peptide; IL2RB; S100A12; CYP1B1; LIPM; E2F2; CD27; ACP5; EPHA2
• ISSN: 0147-6513; 1090-2414
• DOI: 10.1016/j.ecoenv.2021.112668

Ammonia is an acknowledged environment pollutant in atmosphere with irritating smell. Previous studies have shown that excessive ammonia has toxic effects on farm animals and humans. However, the detail toxicity mechanism of ammonia to pigs is still unknown so far. In order to clarify the mechanism of ammonia toxicity, we established a porcine exogenous ammonia poisoning model and assessed the effects of ammonia on the gut-brain axis by transcriptome sequencing, histological observation and chemical analysis. Our results showed that after 30 d of ammonia exposure, 578 differentially expressed genes (DEGs) and 407 DEGs were obtained in the hypothalamus and jejunum, respectively. These DEGs were enriched into Gene Ontology terms associated with inflammation, oxidative metabolism, apoptosis, and the highly expressed genes among these DEGs were verified by real-time quantitative PCR. The content of glutathione and the activities of glutathione peroxidase and superoxide dismutase were significantly decreased, while malondialdehyde content was increased after ammonia exposure. Corticotropin releasing factor, substance P, 5-hydroxytryptamine and ghrelin contents in serum elevated significantly. Furthermore, pathologic observation in the ammonia group revealed infiltration of lymphocytes in the hypothalamus and significant decrease of jejunal epithelial cells. Our results indicated that ammonia exposure mediated changes in transcriptional profiles, pathological damage, oxidative stress and brain-gut peptide of the pig jejunum and hypothalamus, and induced the imbalance of the brain-gut axis through the “oxidative stress-inflammation-apoptosis” interaction network. Our study not only provides a new perspective for the toxicity assessment of ammonia, but also enriches the toxicology mechanism of ammonia. [Display omitted] •Ammonia caused the change of transcriptional profiling in pig gut-brain axis.•Ammonia induced oxidative stress, inflammation and apoptosis in gut-brain axis.•Ammonia exposure caused changes in serum brain-gut peptide.•Ammonia exposure resulted in imbalance of the gut-brain axis.