Marine halophyte derived polyphenols inhibit glioma cell growth through mitogen-activated protein kinase signaling pathway

• Published in: Biomedicine & pharmacotherapy Vol. 159; p. 114288
• Main Authors: Murugesan, Monica, Kandhavelu, Meenakshisundaram, Thiyagarajan, Ramesh, Natesan, Sankar, Rajendran, Priyatharsini, Murugesan, Akshaya
• Format: Journal Article
• Language: English
• Published: France Elsevier Masson SAS 01.03.2023; Elsevier
• Subjects: Antineoplastic Agents - pharmacology; Antioxidants - chemistry; Apoptosis; Cell Proliferation; Flavonoids - analysis; Flavonoids - pharmacology; Glioblastoma multiforme; Glioma - drug therapy; Halophytes; Humans; MAPkinase; Mitogen-Activated Protein Kinases - metabolism; Phenols - analysis; Phenols - pharmacology; Plant Extracts - chemistry; Plant Extracts - pharmacology; Polyphenols; Polyphenols - analysis; Polyphenols - pharmacology; Proteomics; Reactive Oxygen Species - metabolism; Salt-Tolerant Plants - metabolism; Secondary metabolites; Signal Transduction; Polyphenols; Secondary metabolites; MAPkinase; Halophytes; Glioblastoma multiforme; Apoptosis
• ISSN: 0753-3322; 1950-6007
• DOI: 10.1016/j.biopha.2023.114288

Plants that are pharmacologically significant require intensive phytochemical characterization for bioactive profiling of the compounds, which has enabled their safe use in ayurvedic medicine. The present study is focused on the phytochemical analyses, quantitative estimation and profiling of secondary metabolites of leaf extract, as well as the antioxidant and cytotoxic activity of the potent halophytes such as Avicennia marina, Ceriops tagal, Ipomoea pes-caprae, and Sonneratia apetala. The in vitro antioxidant property was investigated using DPPH, ferric reducing antioxidant capacity (FRAP) assay. Bioactive compounds such as phenols, flavonoids, saponin and alkaloids were quantitatively estimated from the extracts of A.marina, C.tagal, I.pes-capra and S.apetala, which possessed higher phenol content than the other studied halophytes. The extracts at 200 µg/ml revealed higher antioxidant activity than the standard ascorbic acid and it functions as a powerful oxygen free radical scavenger with 77.37%, 75.35% and 72.84% for S.apetala, I.pes-caprae and C.tagal respectively and with least IC50 for I.pes-caprae (11.95 µg/ml) followed by C.tagal (49.94 µg/ml). Cell viability and anti-proliferative activity of different polyphenolic fractions of C.tagal (CT1 and CT2) and I.pes-caprae fraction (IP) against LN229, SNB19 revealed Ipomoea as the promising anti-cytotoxic fraction. IP-derived polyphenols was further subjected to apoptosis, migration assay, ROS and caspase − 3 and − 7 to elucidate its potentiality as a therapeutic drug. IP-polyphenols was found to have higher percentage of inhibition than the CT1 and CT2 polyphenols of C.tagal on comparison with TMZ. All the above-mentioned in-vitro analysis further validated the ability of IP-polyphenols inducing cell death via ROS-mediated caspase dependent pathway. Further, proteomic and phospho-proteomic analysis revealed the potential role of IP-polyphenols in the regulation of cell proliferation through MMK3, p53, p70 S6 kinase and RSK1 proteins involved in mitogen-activated protein kinase signaling pathway. Our analysis confirmed the promising role of I.pes-caprae derived polyphenols as an anti-metastatic compound against GBM cells. [Display omitted] •Ipomoea pes-caprae extract was rich in polyphenol.•Ipomoea pes-caprae (IP) extract exhibits anti-proliferative and anti-metastatic properties in GBM cells.•Ipomoea pes-caprae metabolites induce cell death via ROS-mediated caspase dependent pathway.•Ipomoea pes-caprae extract induce phosphorylation of MMK3, p53, p70 S6 kinase and RSK1 proteins.•Ipomoea pes-caprae extract function as the modulator of MAPkinase signaling pathway in GBM cells.