Genetic differentiation and efficient sex-specific marker development of a pair of Y- and X-linked markers in yellow catfish

Pf62-Y and Pf62-X is a pair of allelic Y chromosome-linked and X chromosome-linked markers, and have been used to identify YY super-males, XY males and XX females for commercial production of all-male populations in yellow catfish (Pelteobagrus fulvidraco). However, the SCAR primers used previously...

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Published inInternational journal of biological sciences Vol. 9; no. 10; pp. 1043 - 1049
Main Authors Dan, Cheng, Mei, Jie, Wang, Da, Gui, Jian-Fang
Format Journal Article
LanguageEnglish
Published Australia Ivyspring International Publisher 01.01.2013
Subjects
Animals
Aquaculture
Catfishes - genetics
Chromosome Walking
Female
Genetic Linkage
Genetic Markers
Male
Pelteobagrus fulvidraco
Research Paper
Sex Factors
X Chromosome
Y Chromosome
All-male fish
Yellow catfish
YY super-males
Sex-specific marker
genome walking
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Summary:Pf62-Y and Pf62-X is a pair of allelic Y chromosome-linked and X chromosome-linked markers, and have been used to identify YY super-males, XY males and XX females for commercial production of all-male populations in yellow catfish (Pelteobagrus fulvidraco). However, the SCAR primers used previously have only two nucleotide difference, which restricts the wide utility because of nucleotide polymorphism. In this study, a continuous 8102 bp Pf62-Y sequence and a 5362 bp Pf62-X sequence have been cloned by genome walking, and significant genetic differentiation has been revealed between the corresponding X and Y chromosome allele sequences. Moreover, three pairs of primers were designed to efficiently identify YY super-males, XY males and XX females in an artificial breeding population, and to distinguish XY males and XX females in various wild populations. Together, the three new sex-specific genetic markers develop a highly stable and efficient method for genetic sex identification and sex control application in sustainable aquaculture of all-male yellow catfish.
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Competing Interests: The authors have declared that no competing interest exists.
ISSN:1449-2288
1449-2288
DOI:10.7150/ijbs.7203