HPLC-ESI- MS/MS analysis of beet (Beta vulgaris) leaves and its beneficial properties in type 1 diabetic rats

• Published in: Biomedicine & pharmacotherapy Vol. 120; p. 109541
• Main Authors: Abd El-Ghffar, Eman A., Hegazi, Nesrine M., Saad, Hamada H., Soliman, Mohamed M., El-Raey, Mohamed A., Shehata, Safia M., Barakat, Alaa, Yasri, Aziz, Sobeh, Mansour
• Format: Journal Article
• Language: English
• Published: France Elsevier Masson SAS 01.12.2019; Elsevier
• Subjects: Animals; Antioxidants - metabolism; Atherosclerosis - blood; Beta vulgaris; Beta vulgaris - chemistry; Blood Glucose - metabolism; Body Weight - drug effects; C-glycosyl flavonoids; Chromatography, High Pressure Liquid; Cytokines; Cytokines - metabolism; Diabetes Mellitus, Experimental - blood; Diabetes Mellitus, Experimental - drug therapy; Diabetes Mellitus, Type 1 - blood; Diabetes Mellitus, Type 1 - drug therapy; Female; Glutathione - metabolism; Humans; Inflammation Mediators - metabolism; Lipids - blood; Liver - metabolism; Male; Malondialdehyde - metabolism; Molecular Docking Simulation; Molecular networking; Nuclear factor kappa B; Oxidative stress; Phenols - analysis; Phytochemicals - analysis; Phytotherapy; Plant Extracts - pharmacology; Plant Extracts - therapeutic use; Plant Leaves - chemistry; PPAR gamma - metabolism; PPAR-ɣ; Rats, Wistar; Spectrometry, Mass, Electrospray Ionization; Tandem Mass Spectrometry; Toxicity Tests, Acute; Molecular networking; Oxidative stress; C-glycosyl flavonoids; Cytokines; Beta vulgaris; PPAR-ɣ; Nuclear factor kappa B
• ISSN: 0753-3322; 1950-6007
• DOI: 10.1016/j.biopha.2019.109541

[Display omitted] The phenolic profile of the leaves of Beta vulgaris subspecies vulgaris variety rubra was investigated by high-performance liquid chromatography (HPLC) coupled to electrospray ionization high resolution mass spectrometric (ESI-HRMS-MS) detection. Mass spectrometry-based molecular networking was employed to dereplicate the known compounds. Twelve known compounds, seven of which are previously undescribed as constituents in the B. vulgaris leaves were dereplicated and assigned with various levels of identification confidence. The ameliorative effects of the aqueous methanolic extract of the leaves were assessed against alloxan induced diabetic rats. It was found that the extract significantly decreased (p < 0.001) serum glucose, lipid profile, ALT, AST, TNF-α, IL-1β, IL-6, and hepatic MDA levels; and significantly increased (p < 0.001) hepatic TAO and GSH; and down-regulated the expression of hepatic NF-κB versus the untreated diabetic groups, in a dose-dependent manner. In molecular docking, all identified compounds exhibited good glide score against the PPAR-ɣ target, confirming the in vivo observed activities. In conclusion, B. vulgaris has immunomodulatory / antioxidant effects that could be helpful in slowing the progression of diabetic complications.