Oral treatment with T6-loaded yeast cell wall particles reduces the parasitemia in murine visceral leishmaniasis model
Yeast cell wall particles isolated from Saccharomyces cerevisiae ( sc YCWPs) have a rich constitution of β-glucan derived from the cell wall. After removing intracellular contents, β-glucan molecules are readily recognized by dectin-1 receptors, present on the cytoplasmic membrane surface of the mon...
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Published in | Scientific reports Vol. 9; no. 1; pp. 20080 - 10 |
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Main Authors | , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
London
Nature Publishing Group UK
27.12.2019
Nature Publishing Group |
Subjects | |
Online Access | Get full text |
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Summary: | Yeast cell wall particles isolated from
Saccharomyces cerevisiae
(
sc
YCWPs) have a rich constitution of β-glucan derived from the cell wall. After removing intracellular contents, β-glucan molecules are readily recognized by dectin-1 receptors, present on the cytoplasmic membrane surface of the mononuclear phagocytic cells and internalized.
Leishmania
spp. are obligate intracellular parasites; macrophages are its primary host cells. An experimental murine model of visceral leishmaniasis caused by
L. infantum
was used to evaluate the antileishmanial activity of oral administration of these particles. A low-water soluble thiophene previously studied
in vitro
against
L. infantum
was entrapped into
sc
YCWPs to direct it into the host cell, in order to circumvent the typical pharmacokinetic problems of water-insoluble compounds. We found that
sc
YCWPs + T6 reduced the parasitic burden in the liver and spleen. There was an increase in IFN-γ levels related to nitric oxide production, explaining the reduction of the
L. infantum
burden in the tissue. Histological analysis did not show signals of tissue inflammation and biochemical analysis from plasma did not indicate signals of cytotoxicity after
sc
YCWPs + T6 treatment. These findings suggested that
sc
YCWPs + T6 administered through oral route reduced the parasitic burden without causing toxic effects, satisfying requirements for development of new strategies to treat leishmaniasis. |
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ISSN: | 2045-2322 2045-2322 |
DOI: | 10.1038/s41598-019-56647-w |