Rapid Biochemical Mixture Screening by Three-Dimensional Patterned Multifunctional Substrate with Ultra-Thin Layer Chromatography (UTLC) and Surface Enhanced Raman Scattering (SERS)

We present a three-dimensional patterned (3DP) multifunctional substrate with the functions of ultra-thin layer chromatography (UTLC) and surface enhanced Raman scattering (SERS), which simultaneously enables mixture separation, target localization and label-free detection. This multifunctional subs...

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Bibliographic Details
Published inScientific reports Vol. 8; no. 1; p. 516
Main Authors Lee, Bi-Shen, Lin, Pi-Chen, Lin, Ding-Zheng, Yen, Ta-Jen
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group UK 11.01.2018
Nature Publishing Group
Subjects
140/133
142/126
639/301/1005/1009
639/638/11/511
639/638/542/969
82/16
9/10
Chemical reduction
Chromatography
Dendrites
Etching
Humanities and Social Sciences
Localization
multidisciplinary
Nanotechnology
Science
Science (multidisciplinary)
Silver
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Summary:We present a three-dimensional patterned (3DP) multifunctional substrate with the functions of ultra-thin layer chromatography (UTLC) and surface enhanced Raman scattering (SERS), which simultaneously enables mixture separation, target localization and label-free detection. This multifunctional substrate is comprised of a 3DP silicon nanowires array (3DP-SiNWA), decorated with silver nano-dendrites (AgNDs) atop. The 3DP-SiNWA is fabricated by a facile photolithographic process and low-cost metal assisted chemical etching (MaCE) process. Then, the AgNDs are decorated onto 3DP-SiNWA by a wet chemical reduction process, obtaining 3DP-AgNDs@SiNWA multifunctional substrates. With various patterns designed on the substrates, the signal intensity could be maximized by the excellent confinement and concentrated effects of patterns. By using this 3DP-AgNDs@SiNWA substrate to scrutinize the mixture of two visible dyes, the individual target could be recognized and further boosted the Raman signal of target 15.42 times comparing to the un-patterned AgNDs@SiNWA substrate. Therefore, such a three-dimensional patterned multifunctional substrate empowers rapid mixture screening, and can be readily employed in practical applications for biochemical assays, food safety and other fields.
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ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-017-18967-7