Identification and profiling of microRNAs expressed in oral buccal mucosa squamous cell carcinoma of Chinese hamster

• Published in: Scientific reports Vol. 9; no. 1; pp. 15616 - 12
• Main Authors: Xu, Guo-qiang, Li, Li-hong, Wei, Jia-ning, Xiao, Lan-fei, Wang, Xiao-tang, Pang, Wen-biao, Yan, Xiao-yan, Chen, Zhao-yang, Song, Guo-hua
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 30.10.2019; Nature Publishing Group
• Subjects: 38; 38/47; 38/77; 59; 631/208/199; 631/208/69; 631/337/384/331; 631/67/1665/3016; 631/67/69; AKT protein; Animals; Apoptosis - genetics; BAX protein; Bcl-2 protein; Buccal mucosa; Carcinogenesis; Carcinoma, Squamous Cell - genetics; Carcinoma, Squamous Cell - pathology; Caspase-3; Caspase-9; Cricetulus; Gene expression; Gene Expression Profiling; Gene regulation; Humanities and Social Sciences; Immunohistochemistry; Medical research; MicroRNAs; MicroRNAs - genetics; miRNA; Mouth Mucosa - metabolism; Mouth Mucosa - pathology; Mouth Neoplasms - genetics; Mouth Neoplasms - pathology; multidisciplinary; Next-generation sequencing; Oral cancer; Oral squamous cell carcinoma; Phosphatidylinositol 3-Kinases - metabolism; Proto-Oncogene Proteins c-akt - metabolism; PTEN Phosphohydrolase - metabolism; PTEN protein; Science; Science (multidisciplinary); Signal Transduction; Squamous cell carcinoma
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-019-52197-3

MicroRNAs are known to play essential role in the gene expression regulation in cancer. In our research, next-generation sequencing technology was applied to explore the abnormal miRNA expression of oral squamous cell carcinoma (OSCC) in Chinese hamster. A total of 3 novel miRNAs (Novel-117, Novel-118, and Novel-135) and 11 known miRNAs (crg-miR-130b-3p, crg-miR-142-5p, crg-miR-21-3p, crg-miR-21-5p, crg-miR-542-3p, crg-miR-486-3p, crg-miR-499-5p, crg-miR-504, crg-miR-34c-5p, crg-miR-34b-5p and crg-miR-34c-3p) were identified. We conducted functional analysis, finding that 340 biological processes, 47 cell components, 46 molecular functions were associated with OSCC. Meanwhile the gene expression of Caspase-9, Caspase-3, Bax, and Bcl-2 were determined by qRT-PCR and the protein expression of PTEN and p-AKT by immunohistochemistry. Our research proposed further insights to the profiles of these miRNAs and provided a basis for investigating the regulatory mechanisms involved in oral cancer research.