Trichoderma-secreted anthranilic acid promotes lateral root development via auxin signaling and RBOHF-induced endodermal cell wall remodeling

• Published in: Cell reports (Cambridge) Vol. 43; no. 4; p. 114030
• Main Authors: Chen, Yu, Fu, Yansong, Xia, Yanwei, Miao, Youzhi, Shao, Jiahui, Xuan, Wei, Liu, Yunpeng, Xun, Weibing, Yan, Qiuyan, Shen, Qirong, Zhang, Ruifu
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 23.04.2024; Elsevier
• Subjects: anthranilic acid; Arabidopsis - growth & development; Arabidopsis - metabolism; auxin biosynthesis; auxin signaling; auxin transport; Cell Wall - metabolism; cell wall remodeling; CP: Plants; endodermis; Gene Expression Regulation, Plant; Indoleacetic Acids - metabolism; lateral root development; ortho-Aminobenzoates - metabolism; Plant Roots - growth & development; Plant Roots - metabolism; Reactive Oxygen Species - metabolism; ROS burst; secondary metabolite; Signal Transduction; Trichoderma - growth & development; Trichoderma - metabolism; Trichoderma spp; cell wall remodeling; CP: Plants; ROS burst; auxin biosynthesis; anthranilic acid; auxin signaling; endodermis; Trichoderma spp; auxin transport; lateral root development; secondary metabolite
• ISSN: 2211-1247; 2211-1247
• DOI: 10.1016/j.celrep.2024.114030

Trichoderma spp. have evolved the capacity to communicate with plants by producing various secondary metabolites (SMs). Nonhormonal SMs play important roles in plant root development, while specific SMs from rhizosphere microbes and their underlying mechanisms to control plant root branching are still largely unknown. In this study, a compound, anthranilic acid (2-AA), is identified from T. guizhouense NJAU4742 to promote lateral root development. Further studies demonstrate that 2-AA positively regulates auxin signaling and transport in the canonical auxin pathway. 2-AA also partly rescues the lateral root numbers of CASP1pro:shy2-2, which regulates endodermal cell wall remodeling via an RBOHF-induced reactive oxygen species burst. In addition, our work reports another role for microbial 2-AA in the regulation of lateral root development, which is different from its better-known role in plant indole-3-acetic acid biosynthesis. In summary, this study identifies 2-AA from T. guizhouense NJAU4742, which plays versatile roles in regulating plant root development. [Display omitted] •2-AA identified from T. guizhouense NJAU4742 can promote lateral root development•2-AA regulates auxin signaling and transport in the canonical auxin pathway•2-AA enhances endodermal cell wall remodeling via an RBOHF-induced ROS burst Chen et al. show that 2-AA, a compound identified from T. guizhouense NJAU4742, promotes plant lateral root development via the canonical auxin pathway as a stimulator and increases ROS deposition in the Casparian strip as an IAA mimic, which finally enhances endodermal cell wall remodeling and facilitates lateral root emergence.