Structural insights into the role of GTPBP10 in the RNA maturation of the mitoribosome

Abstract Mitochondria contain their own genetic information and a dedicated translation system to express it. The mitochondrial ribosome is assembled from mitochondrial-encoded RNA and nuclear-encoded ribosomal proteins. Assembly is coordinated in the mitochondrial matrix by biogenesis factors that...

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Published inNature communications Vol. 14; no. 1; p. 7991
Main Authors Nguyen, Thu Giang, Ritter, Christina, Kummer, Eva
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group 02.12.2023
Nature Publishing Group UK
Nature Portfolio
Subjects
Assembly
Bacteria
Biosynthesis
Maturation
Mitochondria
Protein synthesis
Proteins
Ribonucleic acid
Ribosomal proteins
Ribosomes
RNA
rRNA
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Summary:Abstract Mitochondria contain their own genetic information and a dedicated translation system to express it. The mitochondrial ribosome is assembled from mitochondrial-encoded RNA and nuclear-encoded ribosomal proteins. Assembly is coordinated in the mitochondrial matrix by biogenesis factors that transiently associate with the maturing particle. Here, we present a structural snapshot of a large mitoribosomal subunit assembly intermediate containing 7 biogenesis factors including the GTPases GTPBP7 and GTPBP10. Our structure illustrates how GTPBP10 aids the folding of the ribosomal RNA during the biogenesis process, how this process is related to bacterial ribosome biogenesis, and why mitochondria require two biogenesis factors in contrast to only one in bacteria.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:2041-1723
2041-1723
DOI:10.1038/s41467-023-43599-z