Muricauda brasiliensis sp. nov., isolated from a mat-forming cyanobacterial culture

• Published in: Brazilian journal of microbiology Vol. 52; no. 1; pp. 325 - 333
• Main Authors: Vizzotto, Carla Simone, Peixoto, Julianna, Green, Stefan Joshua, Lopes, Fabyano Alvares C., Ramada, Marcelo Henrique S., Pires Júnior, Osmindo R., Pinto, Otávio Henrique B., Tótola, Marcos Rogério, Thompson, Fabiano Lopes, Krüger, Ricardo Henrique
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.03.2021; Springer Nature B.V
• Subjects: Appendages; Biomedical and Life Sciences; Catalase; Cell culture; Deoxyribonucleic acid; DNA; Environmental Microbiology - Research Paper; Fatty acids; Food Microbiology; Gene sequencing; Gram-negative bacilli; Life Sciences; Lipids; Medical Microbiology; Microbial Ecology; Microbial Genetics and Genomics; Microbial mats; Microbiology; Microorganisms; Muricauda; Mycology; New species; Phosphatidylethanolamine; Phylogeny; Quinones; rRNA 16S; Sodium chloride; Flavobacteriaceae; Cyanobacterial culture; Abrolhos reef bank; Muricauda brasiliensis
• ISSN: 1517-8382; 1678-4405
• DOI: 10.1007/s42770-020-00400-3

Strain K001 was isolated from a cyanobacterial culture derived from Abrolhos, a reef bank microbial mat (South Atlantic Ocean—Brazil). Cells of K001 are Gram stain–negative, catalase and oxidase-positive, non-motile, rod-shaped, and with or without appendages. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain K001 belongs to the genus Muricauda . The highest strain K001 16S rRNA gene identity, ANI, and dDDH, respectively, are with M. aquimarina (98.90%, 79.23, 21.60%), M. ruestringensis (98.20%, 80.82, 23.40%), and M. lutimaris (97.86%, 79.23, 22.70%). The strain grows at 15–37 °C and between 0.5 and 10% NaCl. The major fatty acids of strain K001 are iso-C 15:0 , iso-C 15:1 G, iso-C 17:0 3-OH, and summed feature 3 (C 16:1 ω6c and/or C 16:1 ω7c). The polar lipids are represented by phosphatidylethanolamine, three unidentified aminolipids, and three unidentified polar lipids. The major respiratory quinone is MK-6. The G+C content of the DNA of strain K001 is 41.62 mol%. Based on polyphasic analysis of strain K001, it was identified as a novel representative of the genus Muricauda and was named Muricauda brasiliensis sp. nov. The type strain is K001 (=CBMAI 2315 T  = CBAS 752 T ).