Difference in Presence and Number of CD83+ Dendritic Cells in Patients with Ulcerative Colitis and Crohn’s Disease

• Published in: Scientific reports Vol. 10; no. 1; p. 10055
• Main Authors: Despalatović, Bruna Rošić, Babić, Marija, Bratanić, Andre, Tonkić, Ante, Vilović, Katarina
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 22.06.2020; Nature Publishing Group
• Subjects: 692/308; 692/4020; 692/4020/1503; 692/4020/1503/257; Adolescent; Adult; Aged; Antigens, CD - metabolism; Biopsy; Case-Control Studies; CD83 Antigen; Colitis, Ulcerative - immunology; Colitis, Ulcerative - pathology; Crohn Disease - immunology; Crohn Disease - pathology; Crohn's disease; Dendritic cells; Dendritic Cells - immunology; Enterocytes; Female; Humanities and Social Sciences; Humans; Immunoglobulins - metabolism; Inflammation; Inflammatory bowel disease; Male; Membrane Glycoproteins - metabolism; Middle Aged; multidisciplinary; Science; Science (multidisciplinary); Ulcerative colitis; Young Adult
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-020-67149-5

Different pathophysiological models provide insight into the important role of CD83 + dendritic cells (DCs) in the pathogenesis of Crohn’s disease (CD) and ulcerative colitis (UC). There were 154 subjects included in this study: 60 with UC, 19 with CD and 75 in the control group. Colonic biopsy was performed in all subjects. Specimens were incubated with a primary anti-CD83 antibody. Intraepithelial DCs per 100 enterocytes were counted. The results were analysed according to demographic data, type of IBD and histological inflammation pattern. The odds ratio for CD83 + DCs=0 in the UC group was 3.4 times higher than that in the control group (OR = 3.4; 95% CI: 1.63–7.14; p = 0.001), and the odds ratio for CD83 + DCs ≥1 in the CD group was 5.3 times higher than that in the UC group (OR = 5.3; 95% CI: 1.4–20.2; p = 0.014). The odds ratio for CD83 + DCs=0 in the acute inflammation group was 2.7 times higher than that in the group without inflammation (OR = 2.7; 95% CI: 1.2–5.9; p = 0.011). In the group of patients with CD and acute inflammation (n = 11), there was only one subject without CD83 + DCs (p = 0,024). These results suggest an association of CD83 + DCs with the type of IBD and the histological inflammation pattern.