Label-free Analysis of CD8+ T Cell Subset Proteomes Supports a Progressive Differentiation Model of Human-Virus-Specific T Cells
Pathogens trigger T cells to express distinct sets of effector proteins. To better understand the molecular mechanisms that drive functional specification, we used high-resolution mass spectrometry and label-free protein quantification to measure proteomic differences between the seven largest circu...
Saved in:
Published in | Cell reports (Cambridge) Vol. 19; no. 5; pp. 1068 - 1079 |
---|---|
Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Elsevier Inc
02.05.2017
Elsevier |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Pathogens trigger T cells to express distinct sets of effector proteins. To better understand the molecular mechanisms that drive functional specification, we used high-resolution mass spectrometry and label-free protein quantification to measure proteomic differences between the seven largest circulating human CD8+ T cell subsets. Hierarchical clustering of the proteomes placed naive and CD45RA-expressing effector-type T cells at the extremes of the spectrum, with central memory and other effector memory stages located in between. Prominent differences between the subsets included expression of specific granzymes, signaling proteins, and molecules involved in metabolic regulation and cell adhesion. Remarkably, whereas most of the proteomic relationships between the subsets occurred in linear variations, a small proportion of proteins was regulated only in discrete subsets. The data obtained from this proteome analysis correspond best to a progressive differentiation model in which specific stable traits are gradually acquired during development.
[Display omitted]
•MS reveals a clear proteomic hierarchy for circulatory CD8+ T cell subsets•Protein expression changes in a mainly linear fashion between subsets•A modest proportion of the proteins are expressed in a non-linear fashion•MS reveals a distinct regulation of the metabolome and adhesome between subsets
van Aalderen et al. show that proteomic comparison of CD8+ T cell subsets in the circulation of humans, using high-resolution mass spectrometry, reveals a clear proteomic hierarchy among CD8+ T cells. The mainly linearly changing expression patterns suggest that CD8+ T cell differentiation occurs in a largely gradual fashion. |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 2211-1247 2211-1247 |
DOI: | 10.1016/j.celrep.2017.04.014 |